Trewick S A, Wallis G P, Morgan-Richards M
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand.
Mol Ecol. 2000 Jun;9(6):657-66. doi: 10.1046/j.1365-294x.2000.00905.x.
Most research on the biological effects of Pleistocene glaciation and refugia has been undertaken in the northern hemisphere and focuses on lowland taxa. Using single-strand conformation polymorphism (SSCP) analysis and sequencing of mitochondrial cytochrome oxidase I, we explored the intraspecific phylogeography of a flightless orthopteran (the alpine scree weta, Deinacrida connectens) that is adapted to the alpine zone of South Island, New Zealand. We found that several mountain ranges and regions had their own reciprocally monophyletic, deeply differentiated lineages. Corrected genetic distance among lineages was 8.4% (Kimura 2-parameter [K2P]) / 13% (GTR + I + Gamma), whereas within-lineage distances were only 2.8% (K2P) / 3.2% (GTR + I + Gamma). We propose a model to explain this phylogeographical structure, which links the radiation of D. connectens to Pliocene mountain building, and maintenance of this structure through the combined effects of mountain-top isolation during Pleistocene interglacials and ice barriers to dispersal during glacials.
大多数关于更新世冰川作用和避难所生物效应的研究都在北半球进行，且聚焦于低地分类群。我们利用单链构象多态性（SSCP）分析和线粒体细胞色素氧化酶I测序，探究了一种适应新西兰南岛高山地带的无翅直翅目昆虫（高山岩螽，Deinacrida connectens）的种内系统地理学。我们发现，几个山脉和地区都有各自相互单系、深度分化的谱系。谱系间的校正遗传距离为8.4%（Kimura双参数[K2P]）/ 13%（GTR + I + Gamma），而谱系内距离仅为2.8%（K2P）/ 3.2%（GTR + I + Gamma）。我们提出了一个模型来解释这种系统地理结构，该模型将高山岩螽的辐射与上新世造山运动联系起来，并通过更新世间冰期山顶隔离和冰期期间冰障对扩散的综合作用来维持这种结构。
