Kerwin S C, Lewis D D, Elkins A D, Oliver J L, Hosgood G, Pechman R D, Dial S L, Strain G M
Department of Veterinary Clinical Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
Am J Vet Res. 2000 Jun;61(6):691-8. doi: 10.2460/ajvr.2000.61.691.
To determine whether hyperbaric oxygen treatment (HBOT) would affect incorporation of an autogenous cancellous bone graft in diaphyseal ulnar defects in cats.
12 mature cats.
Bilateral nonunion diaphyseal ulnar defects were created in each cat. An autogenous cancellous bone graft was implanted in 1 ulnar defect in each cat, with the contralateral ulnar defect serving as a nongrafted specimen. Six cats were treated by use of hyperbaric oxygen at 2 atmospheres absolute for 90 minutes once daily for 14 days, and 6 cats were not treated (control group). Bone labeling was performed, using fluorochrome markers. Cats were euthanatized 5 weeks after implanting, and barium sulfate was infused to evaluate vascularization of grafts. Ulnas were evaluated by use of radiography, microangiography, histologic examination, and histomorphometric examination.
Radiographic scores did not differ between treatment groups. Microangiographic appearance of grafted defects was similar between groups, with all having adequate vascularization. Differences were not observed between treated and nontreated groups in the overall histologic appearance of decalcified samples of tissue in grafted defects. Mean distance between fluorescent labels was significantly greater in cats given HBOT than in nontreated cats. Median percentage of bone formation in grafted defects was significantly greater in cats given HBOT.
Hyperbaric oxygen treatment increased the distance between fluorescent labels and percentage of bone formation when incorporating autogenous cancellous bone grafts in induced nonunion diaphyseal ulnar defects in cats, but HBOT did not affect revascularization, radiographic appearance, or qualitative histologic appearance of the grafts.
确定高压氧治疗（HBOT）是否会影响自体松质骨移植在猫尺骨干骺端缺损中的植入情况。
12只成年猫。
在每只猫双侧制造尺骨干骺端骨不连缺损。在每只猫的1个尺骨缺损处植入自体松质骨移植体，对侧尺骨缺损作为未移植样本。6只猫接受2个绝对大气压的高压氧治疗，每天1次，每次90分钟，共14天，另外6只猫不接受治疗（对照组）。使用荧光染料标记进行骨标记。植入后5周对猫实施安乐死，注入硫酸钡以评估移植体的血管化情况。通过X线摄影、微血管造影、组织学检查和组织形态计量学检查对尺骨进行评估。
治疗组之间的X线摄影评分无差异。移植缺损的微血管造影表现两组相似，均具有充足的血管化。在移植缺损处组织脱钙样本的总体组织学外观方面，治疗组和未治疗组之间未观察到差异。接受HBOT的猫荧光标记之间的平均距离显著大于未治疗的猫。接受HBOT的猫移植缺损处骨形成的中位数百分比显著更高。
在猫诱导性尺骨干骺端骨不连缺损中植入自体松质骨移植体时，高压氧治疗增加了荧光标记之间的距离和骨形成百分比，但HBOT不影响移植体的血管化、X线摄影表现或组织学定性外观。
