Smith J S, Wang X Y, Qian J, Hosek S M, Scheithauer B W, Jenkins R B, James C D
Division of Laboratory Genetics, Mayo Clinic, Mayo Foundation, and Mayo Medical School, Rochester, Minnesota, USA.
J Neuropathol Exp Neurol. 2000 Jun;59(6):495-503. doi: 10.1093/jnen/59.6.495.
Activation of the platelet-derived growth factor (PDGF) signaling system has been implicated in the development and malignant progression of diffuse gliomas. Overexpression of PDGF system components, particularly the alpha subtype receptor (PDGFRA), is common in glial tumors, and PDGFRA gene amplification has been reported in glioblastomas. In order to address the incidence of PDGFRA gene amplification in a broad set of diffuse gliomas, we used Southern and fluorescence in situ hybridization (FISH) analyses to examine 167 astrocytic gliomas (20 grade III and 147 grade IV), 41 anaplastic oligodendrogliomas, and 29 anaplastic oligoastrocytomas. PDGFRA gene amplification was identified in 4 anaplastic oligodendrogliomas and in a single case of anaplastic oligoastrocytoma, but in none of the malignant astrocytomas. Each of the 5 tumors with PDGFRA amplification displayed features generally associated with grade IV malignancy in astrocytic tumors. Consequently, our data indicate that this gene alteration is restricted to tumors having oligodendroglial differentiation and highly anaplastic features.
血小板衍生生长因子（PDGF）信号系统的激活与弥漫性胶质瘤的发生发展及恶性进展有关。PDGF系统成分的过表达，尤其是α亚型受体（PDGFRA），在胶质肿瘤中很常见，且在胶质母细胞瘤中已报道有PDGFRA基因扩增。为了研究广泛的弥漫性胶质瘤中PDGFRA基因扩增的发生率，我们采用Southern杂交和荧光原位杂交（FISH）分析，检测了167例星形胶质细胞瘤（20例III级和147例IV级）、41例间变性少突胶质细胞瘤和29例间变性少突星形细胞瘤。在4例间变性少突胶质细胞瘤和1例间变性少突星形细胞瘤中检测到PDGFRA基因扩增，但在恶性星形细胞瘤中均未检测到。5例有PDGFRA扩增的肿瘤均表现出通常与星形细胞瘤IV级恶性相关的特征。因此，我们的数据表明，这种基因改变仅限于具有少突胶质细胞分化和高度间变特征的肿瘤。
