集胞藻PCC 6803菌株的cya2，一个编码鸟苷酸环化酶的原核基因。
Synechocystis strain PCC 6803 cya2, a prokaryotic gene that encodes a guanylyl cyclase.
作者信息
Ochoa De Alda J A, Ajlani G, Houmard J
机构信息
Dynamique des Membranes Végétales, Complexes Protéines-Pigments, CNRS UMR8543, Ecole Normale Supérieure, 75230 Paris Cedex 05, France.
出版信息
J Bacteriol. 2000 Jul;182(13):3839-42. doi: 10.1128/JB.182.13.3839-3842.2000.
Abstract
Synechocystis strain PCC 6803 exhibits similar levels of cyclic AMP (cAMP) and cyclic GMP (cGMP). A thorough analysis of its genome showed that Cya2 (Sll0646) has all the sequence determinants required in terms of activity and purine specificity for being a guanylyl cyclase. Insertional mutagenesis of cya2 caused a marked reduction in cGMP content without altering the cAMP content. Thus, Cya2 represents the first example of a prokaryotic guanylyl cyclase.
摘要
集胞藻PCC 6803菌株中，环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平相似。对其基因组的全面分析表明，Cya2（Sll0646）具备作为鸟苷酸环化酶在活性和嘌呤特异性方面所需的所有序列决定因素。cya2的插入诱变导致cGMP含量显著降低，而cAMP含量未改变。因此，Cya2代表了原核生物鸟苷酸环化酶的首个实例。
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