Herzog A, Yaguchi M, Cabezón T, Corchuelo M C, Petre J, Bollen A
Mol Gen Genet. 1979 Mar 9;171(1):15-22. doi: 10.1007/BF00274010.
The conditionally lethal mutation, 2861 mis, has been mapped inside the ribosomal protein gene cluster at 72 minutes on the Escherichia coli chromosome and was found to cotransduce at 97% with rpsE (S5). The 2861 mis mutation leads to thermosensitivity and impaired assembly in vivo of 30S ribosomal particles at 42 degrees C. The strain carrying the mutation has an altered S 17 ribosomal protein; the mutational alteration involves a replacement of serine by phenylalanine in protein S 17. Spontaneous reversion to temperature independence can restore the normal assembly in vivo of 30 S ribosomal subunits at 42 degrees C and the normal chromatographical behaviour of the S 17 ribosomal protein in vitro. We conclude therefore that the 2861 mis mutation affects the structural gene for protein S 17 (rpsQ).
条件致死突变体2861 mis已被定位在大肠杆菌染色体上72分钟处的核糖体蛋白基因簇内，并且发现它与rpsE（S5）的共转导率为97%。2861 mis突变导致温度敏感性以及在42℃时30S核糖体颗粒在体内组装受损。携带该突变的菌株具有改变的S17核糖体蛋白；突变改变涉及蛋白S17中丝氨酸被苯丙氨酸取代。自发回复到温度不依赖性可恢复42℃时30S核糖体亚基在体内的正常组装以及S17核糖体蛋白在体外的正常色谱行为。因此，我们得出结论，2861 mis突变影响蛋白S17（rpsQ）的结构基因。
