Friedrich R E, Bartel-Friedrich S, Lautenschläger C, Holzhausen H J, Moll R
Mund-Kiefer-Gesichtschirurgie, Universitäts-Krankenhaus Eppendorf.
Mund Kiefer Gesichtschir. 2000 Mar;4(2):125-30. doi: 10.1007/s100060050183.
The extent of radiogenic salivary gland damage depends on the radiation dose, the fractionation, and the localization of the gland in the radiation field. Because extracellular matrix proteins, for example collagen IV, belong to the main components of basement membranes (BM), which are considered to posses cell- and structure-regulating functions, this study set out to describe radiogenic BM changes. The staining profile of collagen IV was studied in 110 female rat mandibular glands depending on age (1 year vs. 1 1/2 years), dosage (2 Gy/d; total dosages 20, 40, or 60 Gy), the radiation field (inside vs. outside), and on the latency period (1/2 year vs. 1 year), using immunohistochemical methods. The stainings were assessed semiquantitatively and differences were evaluated using multivariate analysis. The staining pattern of the polyclonal antibody in rat tissues did not differ from the pattern found in human salivary glands. Collagen IV was detected at variable levels in the glandular and nerve tissue and in vascular walls (negative: adventitia). Irradiated tissues were stained more strongly than non-irradiated, and differences were significant in salivary glands exposed to more than 20 Gy. Age and the latency period had no significant effect on staining. The BM constituent collagen IV showed dose-dependent increasing expression analogous to the salivary gland damage, which could contribute to disturbed cell-matrix interactions following salivary gland radiation exposure. Several tissue structures may be more sensitive to possible scattered radiation. Information on the pretreatment status is mandatory in histopathological studies on the BM of salivary glands.
放射性唾液腺损伤的程度取决于辐射剂量、分次照射情况以及腺体在辐射野中的位置。由于细胞外基质蛋白，例如IV型胶原，是基底膜（BM）的主要成分，而基底膜被认为具有细胞和结构调节功能，因此本研究旨在描述放射性基底膜的变化。采用免疫组织化学方法，在110只雌性大鼠下颌腺中研究了IV型胶原的染色情况，研究因素包括年龄（1岁与1.5岁）、剂量（2 Gy/天；总剂量20、40或60 Gy）、辐射野（内部与外部）以及潜伏期（半年与1年）。对染色进行半定量评估，并使用多变量分析评估差异。大鼠组织中多克隆抗体的染色模式与人类唾液腺中的模式无异。在腺组织、神经组织和血管壁（阴性：外膜）中均检测到不同水平的IV型胶原。照射后的组织染色比未照射的更强，在接受超过20 Gy辐射的唾液腺中差异显著。年龄和潜伏期对染色无显著影响。基底膜成分IV型胶原显示出与唾液腺损伤类似的剂量依赖性表达增加，这可能导致唾液腺辐射暴露后细胞-基质相互作用紊乱。一些组织结构可能对可能的散射辐射更敏感。在唾液腺基底膜的组织病理学研究中，预处理状态的信息是必不可少的。
