Hakim S G, Jacobsen H Ch, Hermes D, Kosmehl H, Lauer I, Nadrowitz R, Sieg P
Department of Maxillofacial Surgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
Clin Oral Investig. 2004 Mar;8(1):30-5. doi: 10.1007/s00784-003-0249-9. Epub 2004 Jan 21.
In order to evaluate the correlation between functional impairment and changes in the expression pattern of immunohistochemical antibodies in the early phase of radiation-induced dysfunction of salivary glands, eight rabbits were scintigraphically examined prior to and 24 h after irradiation with 15 Gy. The parotid glands were studied using HE-staining, Ki-67, alpha-smooth muscle actin (ASMA) and tenascin-C antibodies at every scintigraphic examination. The results demonstrated a significant alteration in the 99mTc-pertechnetate uptake in all irradiated glands. HE-staining showed no relevant impairment of salivary gland tissue in this early phase. Immunohistochemically, we observed a marked re-distribution of ASMA and tenascin-C as well as a reduction of the proliferating rate of acinar cells. This immunohistochemical change correlated with the functional impairment manifested scintigraphically. This study proves the possibility to assess disorders of salivary gland function with immunohistological antibodies as early as 24 h after irradiation and yields the prerequisites to prove the effects of radioprotective agents on salivary gland tissues.
为了评估放射性诱导唾液腺功能障碍早期功能损伤与免疫组化抗体表达模式变化之间的相关性，对8只兔子在15 Gy照射前及照射后24小时进行了闪烁扫描检查。在每次闪烁扫描检查时，使用苏木精-伊红（HE）染色、Ki-67、α-平滑肌肌动蛋白（ASMA）和肌腱蛋白-C抗体对腮腺进行研究。结果显示，所有受照射腺体的高锝酸盐摄取均有显著改变。HE染色显示在这个早期阶段唾液腺组织无相关损伤。免疫组化方面，我们观察到ASMA和肌腱蛋白-C有明显的重新分布，以及腺泡细胞增殖率降低。这种免疫组化变化与闪烁扫描显示的功能损伤相关。本研究证明了早在照射后24小时就有可能用免疫组织学抗体评估唾液腺功能障碍，并为证明放射防护剂对唾液腺组织的作用提供了前提条件。
