Department of Agronomy, Chiayi Agricultural Experiment Station, Taiwan Agricultural Research Institute, Chiayi 60014, Taiwan.
Department of Bio-Industry Communication and Development, National Taiwan University, Taipei 10617, Taiwan.
Int J Mol Sci. 2021 Dec 13;22(24):13365. doi: 10.3390/ijms222413365.
Flash flooding is a major environmental stressor affecting rice production worldwide. DT3 is a drought-tolerant, recurrent parent with a good yield, edible quality, and agronomic traits akin to those of an elite Taiwanese variety, Taiken9 (TK9). Progenies carrying can enhance submergence stress tolerance and can be selected using the marker-assisted backcross (MAB) breeding method. For foreground selection, Sub1A and SubAB1 were utilized as markers on the BCF, BCF, and BCF generations to select the submergence-tolerant gene, . Background selection was performed in the -BCF genotypes, and the percentages of recurrent parent recovery within individuals ranged from 84.7-99.55%. BCF genotypes ( = 100) were evaluated for agronomic traits, yield, and eating quality. Four of the eleven BCF lines showed good yield, yield component, grain, and eating quality. Four BCF lines, SU39, SU40, SU89, and SU92, exhibited desirable agronomic traits, including grain quality and palatability, consistent with those of DT3. These genotypes displayed a high survival rate between 92 and 96%, much better compared with DT3 with 64%, and demonstrated better drought tolerance compared to IR64 and IR96321-345-240. This study provides an efficient and precise MAB strategy for developing climate-resilient rice varieties with good grain quality for flood-prone regions.
突发性洪水是影响全球水稻生产的主要环境胁迫因素。DT3 是一种耐旱、轮回亲本，具有良好的产量、食用品质和农艺性状，类似于台湾优良品种 Taiken9（TK9）。携带 的后代可以增强耐淹没胁迫能力，并可以使用标记辅助回交（MAB）育种方法进行选择。对于前景选择，Sub1A 和 SubAB1 被用作 BCF、BCF 和 BCF 世代的标记，以选择耐淹没基因 。背景选择在 -BCF 基因型中进行，个体内轮回亲本恢复的百分比范围为 84.7-99.55%。对 BCF 基因型（ = 100）进行了农艺性状、产量和食用品质评估。11 个 BCF 系中的 4 个表现出良好的产量、产量构成、粒重和食用品质。SU39、SU40、SU89 和 SU92 这 4 个 BCF 系表现出理想的农艺性状，包括籽粒品质和适口性，与 DT3 一致。这些基因型的存活率在 92%至 96%之间，明显优于 DT3 的 64%，并且比 IR64 和 IR96321-345-240 具有更好的耐旱性。本研究为开发具有良好谷物品质的耐洪水气候水稻品种提供了一种高效、精确的 MAB 策略。
