在三种寄生原生生物中，3'非翻译区和聚腺苷酸尾的组合长度并不控制甘油醛-3-磷酸脱氢酶mRNA的翻译速率。
The length of the combined 3' untranslated region and poly(A) tail does not control rates of glyceraldehyde-3-phosphate dehydrogenase mRNA translation in three species of parasitic protists.
作者信息
ter Kuile B H, Sallés F J
机构信息
The Rockefeller University, New York, New York 10021-6399, USA.
出版信息
J Bacteriol. 2000 Jun;182(12):3587-9. doi: 10.1128/JB.182.12.3587-3589.2000.
Abstract
Experimental observations suggested that the length of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA 3' end has a role in regulating rates of translation in the parasitic protists Trypanosoma brucei, Leishmania donovani, and Trichomonas vaginalis. Using a PCR assay for poly(A) tail length, we measured the size of the RNA 3' end under different growth conditions in all three species. Our results showed that the combined 3' untranslated region and poly(A) tail of GAPDH mRNA do not vary with different rates of translation.
摘要
实验观察表明，甘油醛-3-磷酸脱氢酶（GAPDH）mRNA 3'端的长度在调节寄生原生生物布氏锥虫、杜氏利什曼原虫和阴道毛滴虫的翻译速率中起作用。我们使用针对聚腺苷酸（poly(A)）尾长度的PCR检测方法，测量了这三种物种在不同生长条件下RNA 3'端的大小。我们的结果表明，GAPDH mRNA的3'非翻译区和聚腺苷酸尾的组合不会随不同的翻译速率而变化。
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