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嗜酸专性化能自养亚铁氧化细菌氧化亚铁硫杆菌通过bc(1)和NADH-Q氧化还原酶复合物存在上坡电子转移的首个证据。

First evidence for existence of an uphill electron transfer through the bc(1) and NADH-Q oxidoreductase complexes of the acidophilic obligate chemolithotrophic ferrous ion-oxidizing bacterium Thiobacillus ferrooxidans.

作者信息

Elbehti A, Brasseur G, Lemesle-Meunier D

机构信息

Bioénergétique et Ingénierie des Proteines, CNRS, Institut de Biologie Structurale et Microbiologie, 13402 Marseille, cedex 20, France.

出版信息

J Bacteriol. 2000 Jun;182(12):3602-6. doi: 10.1128/JB.182.12.3602-3606.2000.

DOI:10.1128/JB.182.12.3602-3606.2000
PMID:10852897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC101977/
Abstract

The energy-dependent electron transfer pathway involved in the reduction of pyridine nucleotides which is required for CO(2) fixation to occur in the acidophilic chemolithotrophic organism Thiobacillus ferrooxidans was investigated using ferrocytochrome c as the electron donor. The experimental results show that this uphill pathway involves a bc(1) and an NADH-Q oxidoreductase complex functioning in reverse, using an electrochemical proton gradient generated by ATP hydrolysis. Based on these results, a model is presented to explain the balance of the reducing equivalent from ferrocytochrome c between the exergonic and endergonic electron transfer pathways.

摘要

以亚铁细胞色素c作为电子供体，对嗜酸性化能自养生物氧化亚铁硫杆菌中二氧化碳固定所需的吡啶核苷酸还原过程中涉及的能量依赖性电子传递途径进行了研究。实验结果表明，这种上坡途径涉及一个bc(1)复合物和一个以反向方式起作用的NADH-Q氧化还原酶复合物，它们利用ATP水解产生的电化学质子梯度。基于这些结果，提出了一个模型来解释来自亚铁细胞色素c的还原当量在放能和吸能电子传递途径之间的平衡。

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