Boehm M K, Perkins S J
Department of Biochemistry and Molecular Biology, Royal Free and University College Medical School, University College London, London, UK.
FEBS Lett. 2000 Jun 9;475(1):11-6. doi: 10.1016/s0014-5793(00)01612-4.
MFE23 is a single chain Fv antibody that has a high affinity for carcinoembryonic antigen (CEA). A full homology model for CEA based on V-type, I-type and C2-type immunoglobulin folds, 28 oligosaccharides and the interdomain angle of CD2 was validated using solution scattering data. The superimposition of the intermolecular contacts observed in our recent crystal structure of MFE23 with the N-terminal domain of CEA permitted the MFE23-CEA complex to be modelled. Good surface and electrostatic complementarity and carbohydrate-unhindered access of MFE23 with the indentation between the first two CEA domains was observed. The model is supported by biochemical data and provides insight on the high affinity of MFE23 for CEA.
MFE23是一种对癌胚抗原（CEA）具有高亲和力的单链Fv抗体。基于V型、I型和C2型免疫球蛋白折叠、28种寡糖以及CD2的结构域间角度构建的CEA完整同源模型，利用溶液散射数据进行了验证。通过将我们最近获得的MFE23晶体结构中观察到的分子间接触与CEA的N端结构域进行叠加，构建了MFE23-CEA复合物模型。观察到MFE23具有良好的表面和静电互补性，并且能够不受碳水化合物阻碍地进入CEA前两个结构域之间的凹陷处。该模型得到了生化数据的支持，并为MFE23对CEA的高亲和力提供了见解。
