Ralph P, Broxmeyer H E, Nakoinz I
J Exp Med. 1977 Aug 1;146(2):611-6. doi: 10.1084/jem.146.2.611.
Monocyte tumor cell line PU5-1.8 does not normally produce colony-stimulating activity (CSA) required by granulocyte and macrophage progenitors to proliferate and mature in agar. However, CSA is induced in the culture line by as little as 10 ng/ml endotoxic lipopolysaccharide (LPS), with maximum CSA production and release to the medium between 2 and 3 days of incubation. Derived lipid A, but not alkali-treated LPS, is also active. Induction requires RNA and protein synthesis, but is not blocked by mitomycin C or Colcemid. Other inducers of CSA include Mycobacterium Bacillus Calmette-Guérin, tuberculin protein preparation purified protein derivative, zymosan, and phorbol myristate. All inducing agents are specific inhibitors of the monocyte tumor cell proliferation in vitro. Latex beads, another macrophage-activating agent, are rapidly phagocytosed by PU5-1.8 cells, but neither inhibit growth nor induce CSA.
单核细胞肿瘤细胞系PU5-1.8通常不会产生粒细胞和巨噬细胞祖细胞在琼脂中增殖和成熟所需的集落刺激活性（CSA）。然而，低至10 ng/ml的内毒素脂多糖（LPS）可在该培养细胞系中诱导产生CSA，孵育2至3天时CSA产量最高并释放到培养基中。衍生的脂质A具有活性，而碱处理的LPS则无活性。诱导需要RNA和蛋白质合成，但不受丝裂霉素C或秋水仙酰胺的阻断。CSA的其他诱导剂包括卡介苗、结核菌素蛋白制剂纯化蛋白衍生物、酵母聚糖和佛波醇肉豆蔻酸酯。所有诱导剂都是单核细胞肿瘤细胞体外增殖的特异性抑制剂。另一种巨噬细胞激活剂乳胶珠可被PU5-1.8细胞迅速吞噬，但既不抑制生长也不诱导CSA。
