在二氧化碳校准过程中出现设备故障后，体外受精率和卵裂率降低。
Decreased in vitro fertilization and cleavage rates after an equipment error during CO(2) calibration.
作者信息
Wang H, Wang R, Liu J
机构信息
Clinical Research Unit for Reproductive Medicine, Shanxi Provincial Hospital, Taiyuan, People's Republic of China.
出版信息
Fertil Steril. 2000 Jun;73(6):1247-9. doi: 10.1016/s0015-0282(00)00537-9.
OBJECTIVE
To report the decreased IVF rate of oocytes and the reduced cleavage rate of pronuclear stage embryos after gas has backflowed from a gas analyzer into an incubator during the calibration of the CO(2) concentration.
DESIGN
Case report.
SETTING
Clinical research unit for reproductive medicine in a hospital.
INTERVENTION(S): Backflow of gas from a gas analyzer into an incubator during calibration of the CO(2) concentration.
MAIN OUTCOME MEASURE
The IVF rate of oocytes and the cleavage rate of pronuclear stage embryos.
RESULT(S): Thirty-two oocytes were retrieved from four infertile female patients. Nine oocytes were fertilized and four fertilized oocytes were cleaved. In a related animal experiment, the cleavage rate was 57.1% (36/63) in the control group and 25.4% (16/63) in the study group (P =.00026).
CONCLUSION(S): The backflow of gas from the gas analyzer adversely affected the fertilization of oocytes and the cleavage of pronuclear stage embryos.
目的
报告在二氧化碳浓度校准期间，气体分析仪中的气体回流至培养箱后，卵母细胞体外受精（IVF）率降低以及原核期胚胎卵裂率下降的情况。
设计
病例报告。
单位
一家医院的生殖医学临床研究单位。
干预措施
在二氧化碳浓度校准期间，气体从气体分析仪回流至培养箱。
主要观察指标
卵母细胞的体外受精率和原核期胚胎的卵裂率。
结果
从4名不孕女性患者中获取了32枚卵母细胞。9枚卵母细胞受精，其中4枚受精卵发生卵裂。在一项相关动物实验中，对照组的卵裂率为57.1%（36/63），研究组为25.4%（16/63）（P = 0.00026）。
结论
气体分析仪中的气体回流对卵母细胞的受精和原核期胚胎的卵裂产生了不利影响。
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