在二氧化碳校准过程中出现设备故障后，体外受精率和卵裂率降低。

Decreased in vitro fertilization and cleavage rates after an equipment error during CO(2) calibration.

作者信息

Wang H, Wang R, Liu J

机构信息

Clinical Research Unit for Reproductive Medicine, Shanxi Provincial Hospital, Taiyuan, People's Republic of China.

出版信息

Fertil Steril. 2000 Jun;73(6):1247-9. doi: 10.1016/s0015-0282(00)00537-9.

DOI:10.1016/s0015-0282(00)00537-9

PMID:10856493

Abstract

OBJECTIVE

To report the decreased IVF rate of oocytes and the reduced cleavage rate of pronuclear stage embryos after gas has backflowed from a gas analyzer into an incubator during the calibration of the CO(2) concentration.

DESIGN

Case report.

SETTING

Clinical research unit for reproductive medicine in a hospital.

INTERVENTION(S): Backflow of gas from a gas analyzer into an incubator during calibration of the CO(2) concentration.

MAIN OUTCOME MEASURE

The IVF rate of oocytes and the cleavage rate of pronuclear stage embryos.

RESULT(S): Thirty-two oocytes were retrieved from four infertile female patients. Nine oocytes were fertilized and four fertilized oocytes were cleaved. In a related animal experiment, the cleavage rate was 57.1% (36/63) in the control group and 25.4% (16/63) in the study group (P =.00026).

CONCLUSION(S): The backflow of gas from the gas analyzer adversely affected the fertilization of oocytes and the cleavage of pronuclear stage embryos.

摘要