Nair S, Schreiber E, Thong K L, Pang T, Altwegg M
Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia.
J Microbiol Methods. 2000 Jun;41(1):35-43. doi: 10.1016/s0167-7012(00)00148-2.
Amplified fragment length polymorphism (AFLP) is a recently developed, PCR-based high resolution fingerprinting method that is able to generate complex banding patterns which can be used to delineate intraspecific genetic relationships among bacteria. In the present study, AFLP was evaluated for its usefulness in the molecular typing of Salmonella typhi in comparison to ribotyping and pulsed-field gel electrophoresis (PFGE). Six S. typhi isolates from diverse geographic areas (Malaysia, Indonesia, India, Chile, Papua New Guinea and Switzerland) gave unique, heterogeneous profiles when typed by AFLP, a result which was consistent with ribotyping and PFGE analysis. In a further study of selected S. typhi isolates from Papua New Guinea which caused fatal and non-fatal disease previously shown to be clonally related by PFGE, AFLP discriminated between these isolates but did not indicate a linkage between genotype with virulence. We conclude that AFLP (discriminatory index=0.88) has a higher discriminatory power for strain differentiation among S. typhi than ribotyping (DI=0.63) and PFGE (DI=0.74).
扩增片段长度多态性（AFLP）是一种最近开发的基于聚合酶链反应（PCR）的高分辨率指纹图谱技术，能够产生复杂的条带模式，可用于描绘细菌种内的遗传关系。在本研究中，将AFLP与核糖体分型和脉冲场凝胶电泳（PFGE）相比，评估其在伤寒沙门氏菌分子分型中的实用性。来自不同地理区域（马来西亚、印度尼西亚、印度、智利、巴布亚新几内亚和瑞士）的6株伤寒沙门氏菌分离株经AFLP分型时呈现出独特的、异质性的图谱，这一结果与核糖体分型和PFGE分析一致。在对先前经PFGE显示为克隆相关的巴布亚新几内亚伤寒沙门氏菌分离株进行的进一步研究中，AFLP能够区分这些分离株，但未显示基因型与毒力之间的联系。我们得出结论，AFLP（鉴别指数=0.88）在区分伤寒沙门氏菌菌株方面比核糖体分型（DI=0.63）和PFGE（DI=0.74）具有更高的鉴别力。
