Kellum J M, Jaffe B M
Ann Surg. 1976 Nov;184(5):633-6. doi: 10.1097/00000658-197611000-00018.
Cannulas were placed in the portal vein, hepatic vein, and infrarenal vena cava in eight anesthetized dogs. The duodenum of each dog was irrigated with saline (control) and 0.1 N HCI (50 ml in 10 min). Heparinized blood samples were taken from each cannula 5 min before, and 1, 5, 10, 30 and 60 min after each irrigation for measurement of immunoreactive serotonin concentrations. Serotonin concentrations did not change during saline irrigation of the duodenum. In contrast, serotonin release was consistently observed after duodenal acidification (pH 1.5-2.0). Portal venous serotonin concentrations were increased at 1 min (356 +/- 147 ng/ml) and following a biphasic pattern remained elevated at 30 and 60 min (499 +/- 131 and 489 +/- 187 ng/ml, respectively). Concentrations in the hepatic vein rose more slowly and to a lower peak (357 +/- 123 ng/ml at 10 min). Caval serotonin concentrations were increased at 10 min (342 +/- 121 ng/ml) but promptly returned to baseline levels. In the canine gastrointestinal tract, immunoreactive serotonin concentrations were highest in the duodenal bulb (15.4 mug/gm). This study demonstrated serotonin release from the duodenum following acid perfusion and documented that some of the released serotonin escaped hepatic inactivation. These findings support the possibility that serotonin may be a gastrointestinal hormone involved in the feedback inhibition of gastric acid secretion.
在八只麻醉犬身上，将套管分别置于门静脉、肝静脉和肾下腔静脉。每只犬的十二指肠用生理盐水（对照）和0.1N盐酸（10分钟内50毫升）进行灌洗。在每次灌洗前5分钟以及灌洗后1、5、10、30和60分钟，从每个套管采集肝素化血样，以测量免疫反应性血清素浓度。在十二指肠用生理盐水灌洗期间，血清素浓度没有变化。相反，在十二指肠酸化（pH 1.5 - 2.0）后持续观察到血清素释放。门静脉血清素浓度在1分钟时升高（356±147纳克/毫升），并呈双相模式，在30和60分钟时仍保持升高（分别为499±131和489±187纳克/毫升）。肝静脉中的浓度上升较慢，峰值较低（10分钟时为357±123纳克/毫升）。腔静脉血清素浓度在10分钟时升高（342±121纳克/毫升），但迅速恢复到基线水平。在犬的胃肠道中，免疫反应性血清素浓度在十二指肠球部最高（15.4微克/克）。本研究证明了酸灌注后十二指肠释放血清素，并记录到一些释放的血清素逃脱了肝脏的失活作用。这些发现支持了血清素可能是参与胃酸分泌反馈抑制的胃肠激素这一可能性。
