Damiens C, Grimaud E, Rousselle A V, Charrier C, Fortun Y, Heymann D, Padrines M
Laboratorie de physiopathologie de la résorption osseuse et mécanismes de cicatrisation, Faculté de Chirurgie Dentaire, Nantes, France.
Cytokine. 2000 May;12(5):539-42. doi: 10.1006/cyto.1999.0593.
The production of cysteine protease by two human osteosarcoma cell lines (MG-63 and SaOS2) was analyzed, as well as their modulation by interleukin 1beta (hIL-1 beta), interleukin 6 (hIL-6), insulin growth factor-1 (hIGF-1), oncostatin M (hOSM), leukemia inhibitory factor (hLIF) and growth hormone (hGH). Cysteine protease activities were detected using a synthetic substrate. The protease activities (especially cathepsin L activity) of both cell lines were increased significantly in the presence of hIL-1 beta, hIL-6 and hOSM. In contrast, hIGF-1 and hGH decreased these activities, and no effect was detectable in the presence of hLIF. The addition of antibodies against the gp-130 chain of the hIL-6 and hOSM receptors totally inhibited the stimulating effect of these two cytokines on cysteine protease activities. In increasing collagen type I degradation, hIL-1beta, hIL-6 and hOSM could be involved in bone resorption, whereas the inhibitory action of hIGF-1 and hGH on collagen type I degradation suggest that this factor could play a role in bone formation.
分析了两种人骨肉瘤细胞系（MG-63和SaOS2）半胱氨酸蛋白酶的产生情况，以及白细胞介素1β（hIL-1β）、白细胞介素6（hIL-6）、胰岛素生长因子-1（hIGF-1）、制瘤素M（hOSM）、白血病抑制因子（hLIF）和生长激素（hGH）对其的调节作用。使用合成底物检测半胱氨酸蛋白酶活性。在hIL-1β、hIL-6和hOSM存在的情况下，两种细胞系的蛋白酶活性（尤其是组织蛋白酶L活性）均显著增加。相比之下，hIGF-1和hGH降低了这些活性，并且在hLIF存在的情况下未检测到任何影响。添加针对hIL-6和hOSM受体gp-130链的抗体完全抑制了这两种细胞因子对半胱氨酸蛋白酶活性的刺激作用。在增加I型胶原蛋白降解方面，hIL-1β、hIL-6和hOSM可能参与骨吸收，而hIGF-1和hGH对I型胶原蛋白降解的抑制作用表明该因子可能在骨形成中发挥作用。
