淋病奈瑟菌主要厌氧诱导外膜蛋白AniA的表达可提供针对正常人血清杀伤作用的保护。
Expression of AniA, the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, provides protection against killing by normal human sera.
作者信息
Cardinale J A, Clark V L
机构信息
Department of Microbiology and Immunology, University of Rochester, Rochester, New York 14642, USA.
出版信息
Infect Immun. 2000 Jul;68(7):4368-9. doi: 10.1128/IAI.68.7.4368-4369.2000.
Abstract
Anaerobically grown Neisseria gonorrhoeae has previously been shown to have elevated serum resistance in the absence of exogenous CMP-N-acetylneuraminic acid or detectable sialylation. We hypothesized that the anaerobically induced gonococcal outer membrane protein AniA might have a role in this phenomenon, as it is the only known gonococcal protein that is absent under aerobic conditions. An N. gonorrhoeae F62 derivative, RUG7035, in which aniA is under control of the tac promoter, was used to examine the effect of AniA expression on serum resistance. In this study, we found that expression of AniA enhanced the serum resistance of N. gonorrhoeae and may account for these earlier observations.
摘要
先前已表明，在没有外源性CMP-N-乙酰神经氨酸或可检测到的唾液酸化的情况下，厌氧培养的淋病奈瑟菌具有增强的血清抗性。我们推测，厌氧诱导的淋球菌外膜蛋白AniA可能在这一现象中起作用，因为它是已知的唯一在有氧条件下不存在的淋球菌蛋白。使用aniA受tac启动子控制的淋病奈瑟菌F62衍生物RUG7035来检测AniA表达对血清抗性的影响。在本研究中，我们发现AniA的表达增强了淋病奈瑟菌的血清抗性，这可能解释了这些早期观察结果。
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