Kohler J J, Pathangey L, Hasona A, Progulske-Fox A, Brown T A
Department of Oral Biology, University of Florida, Gainesville, Florida 32610, USA.
Infect Immun. 2000 Jul;68(7):4370-3. doi: 10.1128/IAI.68.7.4370-4373.2000.
We have previously shown that Salmonella enterica serovar Typhimurium expressing the hagB hemagglutinin gene from Porphyromonas gingivalis can induce primary and recall immune responses in serum and secretions in mice; however, the longevity of memory induced by oral Salmonella carriers has not been adequately demonstrated. In this study, we examined the capacity of mice to mount a recall response 52 weeks after primary immunization. Recall responses were seen in serum immunoglobulin G (IgG) and IgA following boosting at week 52, and in most cases, they were equal to or greater than the primary responses. Significant mucosal IgA recall responses in saliva and vaginal wash were also detected following boosting at week 52. In addition, there was a considerable residual response in secretions at week 51, prior to boosting. These results indicate that oral Salmonella vectors can induce long-term memory to recombinant HagB and are particularly effective at inducing long-lasting mucosal responses as well as at inducing the capacity for mucosal recall responses.
我们之前已经表明，表达牙龈卟啉单胞菌hagB血凝素基因的鼠伤寒沙门氏菌可在小鼠血清和分泌物中诱导初次免疫反应和回忆免疫反应；然而，口服沙门氏菌载体诱导的记忆的持久性尚未得到充分证明。在本研究中，我们检测了小鼠在初次免疫52周后产生回忆反应的能力。在第52周加强免疫后，血清免疫球蛋白G（IgG）和IgA中出现了回忆反应，在大多数情况下，它们等于或大于初次反应。在第52周加强免疫后，唾液和阴道冲洗液中也检测到了显著的黏膜IgA回忆反应。此外，在加强免疫前的第51周，分泌物中存在相当大的残余反应。这些结果表明，口服沙门氏菌载体可诱导对重组HagB的长期记忆，并且在诱导持久的黏膜反应以及诱导黏膜回忆反应的能力方面特别有效。
