Attridge S R, Davies R, LaBrooy J T
Department of Microbiology and Immunology, University of Adelaide, Australia.
Vaccine. 1997 Feb;15(2):155-62. doi: 10.1016/s0264-410x(96)00158-2.
Several strains of Salmonella have been used as vectors for the delivery of Escherichia coli fimbrial proteins to the gut-associated lymphoid tissue (GALT) of the mouse. Plasmids carrying a complementing thyA+ gene, together with genes specifying synthesis of K88 or K99, were introduced into non-reverting thyA Salmonella mutants. The resulting constructs expressed the foreign pilin protein on their surfaces and, provided the vector was able to colonize the GALT, elicited strong serum responses to K88 or K99. These responses were dramatically impaired however, in recipients with pre-existing immunity to the vector strain. Mice initially infected with Salmonella stanley ca 4, 10 or 20 weeks prior to oral administration of S. stanley-K88 showed greatly reduced serum responses to K88 as determined by ELISA. The hypo-responsiveness seen in vector-primed mice could be largely overcome by changing the serotype of the strain subsequently used to deliver the foreign protein.
几种沙门氏菌菌株已被用作载体，将大肠杆菌菌毛蛋白递送至小鼠的肠道相关淋巴组织（GALT）。携带互补thyA +基因以及指定合成K88或K99的基因的质粒被引入非回复性thyA沙门氏菌突变体中。所得构建体在其表面表达外源菌毛蛋白，并且如果载体能够在GALT中定殖，则会引发针对K88或K99的强烈血清反应。然而，在对载体菌株具有预先存在免疫力的受体中，这些反应会显著受损。在口服给予斯坦利沙门氏菌-K88之前约4、10或20周最初感染斯坦利沙门氏菌的小鼠，通过ELISA测定显示对K88的血清反应大大降低。通过改变随后用于递送外源蛋白的菌株的血清型，在载体引发的小鼠中看到的低反应性在很大程度上可以被克服。
