Kössi J, Laato M
Department of Surgery, University of Turku, Finland.
Pathobiology. 2000 Jan-Feb;68(1):29-35. doi: 10.1159/000028112.
Viscose cellulose sponge implants in the rat and fibroblast cultures established from granulation tissue, hypertrophic scar, or keloid were treated with different concentrations of glucose, fructose, galactose, mannose, and sucrose. The concentrations of the above-mentioned sugars in wound fluid and cell culture medium were examined at the termination of experiments by liquid chromatography. Results showed that glucose was present in wound fluid in relatively low levels. In addition to glucose, only mannose was found in wound fluid. On the other hand, cell culture studies showed that virtually all the added sugars were found in cell culture medium. The most prominent exception was the decreased concentration of mannose in keloid fibroblast cultures. In addition, glucose concentration in culture medium of keloid fibroblasts was constantly very low except in mannose-treated cultures where the consumption of glucose was dose-dependently decreased compared to increased mannose concentration. Similarly, increased concentrations of galactose and mannose resulted in dose-dependent lowered consumption of glucose in granulation tissue and hypertrophic scar fibroblasts. These findings suggest that the sugar metabolism may differ in various fibroblast cultures. Further, at least in our wound model, only glucose and mannose are present in wound fluid, and excess sugar is rapidly cleared from wound fluid.
用不同浓度的葡萄糖、果糖、半乳糖、甘露糖和蔗糖处理大鼠体内的粘胶纤维素海绵植入物以及由肉芽组织、肥厚性瘢痕或瘢痕疙瘩建立的成纤维细胞培养物。实验结束时，通过液相色谱法检测伤口液和细胞培养基中上述糖类的浓度。结果显示，伤口液中葡萄糖含量相对较低。除葡萄糖外，伤口液中仅发现了甘露糖。另一方面，细胞培养研究表明，几乎所有添加的糖类都存在于细胞培养基中。最显著的例外是瘢痕疙瘩成纤维细胞培养物中甘露糖浓度降低。此外，瘢痕疙瘩成纤维细胞培养基中的葡萄糖浓度一直很低，除了在甘露糖处理的培养物中，与甘露糖浓度增加相比，葡萄糖的消耗量呈剂量依赖性降低。同样，半乳糖和甘露糖浓度的增加导致肉芽组织和肥厚性瘢痕成纤维细胞中葡萄糖的消耗量呈剂量依赖性降低。这些发现表明，不同成纤维细胞培养物中的糖代谢可能存在差异。此外，至少在我们的伤口模型中，伤口液中仅存在葡萄糖和甘露糖，多余的糖会迅速从伤口液中清除。
