长期运动对大鼠胰腺的影响。

The effect of chronic exercise on the rat pancreas.

作者信息

Minato K, Shiroya Y, Nakae Y, Kondo T

机构信息

Laboratory of Exercise Nutrition, Wayo Women's University, Ichikawa, Chiba, Japan.

出版信息

Int J Pancreatol. 2000 Apr;27(2):151-6. doi: 10.1385/IJGC:27:2:151.

DOI:10.1385/IJGC:27:2:151

PMID:10862514

Abstract

BACKGROUND

We recently demonstrated that chronic physical exercise increases pancreatic protein content and basal amylase secretion. It is unknown whether chronic exercise causes hypertrophy or proliferation of pancreatic acinar cells.

METHODS

Female F344 rats (age, 6 wk) were divided into control (n = 7) and exercise (n = 6) groups. Food consumption was matched between the 2 groups. Rats in the control group were kept sedentary. Rats in the exercise group were exercised for 60 min, 5 d/wk during the experiment. After 8 wk, the pancreas and hindlimb muscles were rapidly excised and weighed. Protein and DNA content and enzyme activity in pancreatic tissue were measured. Pancreatic tissues from control and exercised rats were also prepared for transmission electron microscopy.

RESULTS

Inhibition of growth and hypertrophy of hindlimb muscles were exhibited by the exercise group. In the exercise group, pancreatic wet weight, protein content, and amylase and lipase activities, but not DNA content, were significantly higher than those in the control group. Electron micrographs clearly revealed that acinar cells were hypertrophied and zymogen granules were increased in number in exercised rats.

CONCLUSION

Chronic endurance exercise increases pancreatic weight, protein content and enzyme activity through hypertrophy of acinar cells.

摘要

背景

我们最近证明，长期体育锻炼可增加胰腺蛋白质含量和基础淀粉酶分泌。目前尚不清楚长期锻炼是否会导致胰腺腺泡细胞肥大或增殖。

方法

将雌性F344大鼠（6周龄）分为对照组（n = 7）和运动组（n = 6）。两组的食物摄入量相匹配。对照组大鼠保持 sedentary状态。运动组大鼠在实验期间每周5天、每天60分钟进行锻炼。8周后，迅速切除胰腺和后肢肌肉并称重。测量胰腺组织中的蛋白质和DNA含量以及酶活性。还制备了对照组和运动组大鼠的胰腺组织用于透射电子显微镜检查。