Büchler M, Malfertheiner P, Fischbach W, Beger H G
Eur Surg Res. 1987;19(1):31-9. doi: 10.1159/000128678.
Subtotal colectomy was performed in 17 rats. After 2 or 4 weeks the entire pancreas was excised and isolated pancreatic lobules were incubated for 60 min in Krebs-Ringer-Hepes solution either with or without addition of ceruletide (5 X 10(-10) g/ml). Amylase was determined every 15 min in the supernatant medium. Amylase, trypsin, DNA and total protein were measured in homogenized pancreatic tissue. Morphology was performed via light-microscopy analysis. Two weeks after subtotal colectomy we found an increase in pancreatic wet weight, enzyme and protein content, cellular size and secretion capacity in vitro, whereas DNA concentration in relation to pancreatic tissue weight in grams decreased. These changes pointed to pancreatic hypertrophy. Four weeks after surgery, pancreatic wet weight, enzyme content and DNA content as well as in vitro amylase discharge increased, whereas cellular size remained unchanged compared to controls and sham-operated rats. These changes were indicative of pancreatic hyperplasia.
对17只大鼠实施了结肠次全切除术。2周或4周后，切除整个胰腺，将分离出的胰腺小叶在含有或不添加雨蛙肽（5×10⁻¹⁰克/毫升）的Krebs-Ringer-Hepes溶液中孵育60分钟。每隔15分钟测定上清液培养基中的淀粉酶含量。在匀浆后的胰腺组织中测定淀粉酶、胰蛋白酶、DNA和总蛋白含量。通过光学显微镜分析进行形态学观察。结肠次全切除术后两周，我们发现胰腺湿重、酶和蛋白质含量、细胞大小以及体外分泌能力增加，而相对于以克为单位的胰腺组织重量，DNA浓度降低。这些变化表明胰腺肥大。术后四周，胰腺湿重、酶含量和DNA含量以及体外淀粉酶分泌增加，而与对照组和假手术大鼠相比，细胞大小保持不变。这些变化表明胰腺增生。
