Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, Rizzi M, Steensma HY, Verrips CT, Vindeløv J, Pronk JT
Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
Enzyme Microb Technol. 2000 Jun 1;26(9-10):706-714. doi: 10.1016/s0141-0229(00)00162-9.
To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.
为了选择一种适合（分子）遗传、生理和生化工程研究中所使用实验技术的酿酒酵母参考菌株，我们在四种二倍体、原养型实验室菌株中研究了多种特性。研究了以下参数：1）摇瓶培养中的最大比生长速率；2）在分批培养和稳态恒化器培养中，在控制pH值和溶解氧浓度的条件下，在限定培养基上生长时葡萄糖的生物量产量；3）在葡萄糖限制的恒化器培养中，高于该值时需氧发酵变得明显的临界比生长速率；4）孢子形成和交配效率；5）通过醋酸锂、二羟乙基甘氨酸和电穿孔法的转化效率。基于生理和遗传特性，选择了CEN.PK家族的菌株作为细胞工厂研究生长和产物形成的化学计量学和动力学的平台。
