Dingman C W, Kakunaga T
Int J Radiat Biol Relat Stud Phys Chem Med. 1976 Jul;30(1):55-66. doi: 10.1080/09553007614550801.
We describe a reproducible technique for measuring DNA strand breaking and rejoining in cells after treatment with U.V.-light. Results obtained with normal human cells, xeroderma pigmentosum cells (XP, complementation group A) and XP variant cells suggest that all three of these cell-types can carry out single-strand incision with equal rapidity. However, the breaks so induced appeared to be only slowly rejoined in the XP variant cells and rejoined not at all in XP complementation group A cells. Furthermore, parental strand rejoining was inhibited by caffeine in XP variant cells but not in normal cells.
我们描述了一种可重复的技术，用于测量紫外线处理后细胞中DNA链的断裂和重新连接。用正常人细胞、着色性干皮病细胞（XP，互补组A）和XP变异细胞获得的结果表明，这三种细胞类型都能以相同的速度进行单链切割。然而，如此诱导产生的断裂在XP变异细胞中似乎只是缓慢重新连接，而在XP互补组A细胞中则完全不会重新连接。此外，咖啡因在XP变异细胞中抑制亲本链重新连接，但在正常细胞中则不会。
