Mazur L
Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Mutat Res. 2000 Jun 22;468(1):27-33. doi: 10.1016/s1383-5718(00)00037-1.
The frequency of micronucleated polychromatic erythrocytes (MNPCEs) was assessed in the bone marrow and peripheral blood of adult male Swiss mice treated with reduced glutathione (GSH) and S-2-/3-aminopropylamino/ethyl phosphorothioic acid (WR-2721), at a dose of 400 mg/kg body weight, and exposed to 6 Gy X-rays. GSH or WR-2721 was applied alone, or 60 and 30 min, respectively, prior to X-ray-exposure. The number of MNPCEs was determined at 24 h after the thiol treatment and X-irradiation. The radioprotection and toxicity caused in the mouse erythroblasts by GSH and WR-2721, as indicated by the number of MNPCEs were dependent on the thiol applied. The stronger radioprotective effect is obtained following WR-2721 administration than after GSH application. WR-2721 showed greater toxicity than GSH. The combination of GSH and WR-2721 given before X-ray-exposure resulted in the most radioprotective effect as compared to the respective single-drug treatment of mice. Application of the both thiols, without subsequent X-irradiation appeared to be the most toxic, compared with administration of WR-2721 or GSH alone. The effective radioprotection by the combined action of GSH and WR-2721 against genomic instability induced in the mouse erythroblasts by X-rays was shown.
对成年雄性瑞士小鼠的骨髓和外周血中微核多色红细胞（MNPCEs）的频率进行了评估。这些小鼠以400mg/kg体重的剂量接受还原型谷胱甘肽（GSH）和S-2-/3-氨丙基氨基/乙基硫代磷酸（WR-2721）处理，并暴露于6Gy的X射线下。GSH或WR-2721单独应用，或分别在X射线照射前60分钟和30分钟应用。在硫醇处理和X射线照射后24小时测定MNPCEs的数量。如MNPCEs数量所示，GSH和WR-2721对小鼠成红细胞产生的辐射防护作用和毒性取决于所应用的硫醇。与应用GSH相比，给予WR-2721后获得更强的辐射防护作用。WR-2721显示出比GSH更大的毒性。与对小鼠的各自单药治疗相比，在X射线照射前给予GSH和WR-2721的组合产生了最大的辐射防护作用。与单独给予WR-2721或GSH相比，应用两种硫醇且随后不进行X射线照射似乎毒性最大。显示了GSH和WR-2721联合作用对X射线诱导的小鼠成红细胞基因组不稳定性的有效辐射防护作用。
