Nadi Saba, Monfared Ali Shabestani, Mozdarani Hossein, Mahmodzade Aziz, Pouramir Mahdi
Department of Radiobiology and Radiation Protection, Babol University of Medical Sciences, Babol, Iran.
Cellular & Molecular Biology Research Center, Department of Medical physics, Babol University of Medical Sciences, Babol, Iran.
Iran J Med Sci. 2016 May;41(3):180-5.
Interactions of free radicals from ionizing radiation with DNA can induce DNA damage and lead to mutagenesis and carsinogenesis. With respect to radiation damage to human, it is important to protect humans from side effects induced by ionizing radiation. In the present study, the effects of arbutin were investigated by using the micronucleus test for anti-clastogenic activity, to calculate the ratio of polychromatic erythrocyte to polychromatic erythrocyte plus normochromatic erythrocyte (PCE/PCE+NCE) in order to show cell proliferation activity.
Arbutin (50, 100, and 200 mg/kg) was intraperitoneally (ip)administered to NMRI mice two hours before gamma radiation at 2 and 4 gray (Gy). The frequency of micronuclei in 1000 PCEs (MnPCEs) and the ratio of PCE/PCE+NCE were calculated for each sample. Data were statistically evaluated using one-way ANOVA, Tukey HSD test, and t-test.
The findings indicated that gamma radiation at 2 and 4 Gy extremely increased the frequencies of MnPCE (P<0.001) while reducing PCE/PCE+NCE (P<0.001) compared to the control group. All three doses of arbutin before irradiation significantly reduced the frequencies of MnPCEs and increased the ratio of PCE/PCE+NCE in mice bone marrow compared to the non-drug-treated irradiated control (P<0.001). All three doses of arbutin had no toxicity effect on bone marrow cells. The calculated dose reduction factor (DRF) showed DRF=1.93 for 2Gy and DRF=2.22 for 4 Gy.
Our results demonstrated that arbutin gives significant protection to rat bone against the clastogenic and cytotoxic effects of gamma irradiation.
电离辐射产生的自由基与DNA相互作用可导致DNA损伤，进而引发诱变和致癌作用。对于人类辐射损伤而言，保护人体免受电离辐射的副作用至关重要。在本研究中，通过微核试验研究熊果苷的抗断裂活性，并计算多色红细胞与多色红细胞加正色红细胞的比例（PCE/PCE+NCE）以显示细胞增殖活性。
在2和4格雷（Gy）的γ射线照射前两小时，将熊果苷（50、100和200毫克/千克）腹腔注射（ip）给NMRI小鼠。计算每个样本中1000个多色红细胞中的微核频率（MnPCEs）以及PCE/PCE+NCE的比例。数据采用单因素方差分析、Tukey HSD检验和t检验进行统计学评估。
研究结果表明，与对照组相比，2和4 Gy的γ射线照射极大地增加了MnPCE的频率（P<0.001），同时降低了PCE/PCE+NCE（P<0.001）。与未用药物处理的辐射对照组相比，照射前的所有三个剂量的熊果苷均显著降低了小鼠骨髓中MnPCEs的频率，并增加了PCE/PCE+NCE的比例（P<0.001）。所有三个剂量的熊果苷对骨髓细胞均无毒性作用。计算得出的剂量降低因子（DRF）显示，2 Gy时DRF = 1.93，4 Gy时DRF = 2.22。
我们的结果表明，熊果苷能显著保护大鼠骨骼免受γ射线照射的断裂和细胞毒性作用。
