Inactin concentration in plasma of rats during anaesthesia and the effect of this concentration on short circuit current of isolated frog skin.

Inactin, given to rats for anaesthesia at a dose of 100 mg per kg body weight results in an arterial plasma concentration of 10.6 +/- 1.14 mg%, measured 40 min after the onset of anaesthesia. When similar concentrations of Inactin or Amytal were applied to frog skins the decrease in the in vitro short circuit current is dose dependent. With Inactin, the inhibition was, at least in part, reversible and the electrical conductance was not affected. Amytal however was found to depress SCC and electrical conductance irreversibly. The data are discussed in regard to active renal transport processes.