腺病毒晚期基因表达不需要类似Rev的核RNA输出途径。
Adenovirus late gene expression does not require a Rev-like nuclear RNA export pathway.
作者信息
Rabino C, Aspegren A, Corbin-Lickfett K, Bridge E
机构信息
Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.
出版信息
J Virol. 2000 Jul;74(14):6684-8. doi: 10.1128/jvi.74.14.6684-6688.2000.
Abstract
Adenovirus late mRNA export is facilitated by viral early proteins of 55 and 34 kDa. The 34-kDa protein contains a leucine-rich nuclear export signal (NES) similar to that of the human immunodeficiency virus Rev protein. It was proposed that the 34-kDa protein might facilitate the export of adenovirus late mRNA through a Rev-like NES-mediated export pathway. We have tested the role of NES-mediated RNA export during adenovirus infection, and we find that it is not essential for the expression of adenovirus late genes.
摘要
腺病毒晚期mRNA的输出由55 kDa和34 kDa的病毒早期蛋白促进。34 kDa的蛋白含有一个富含亮氨酸的核输出信号（NES），类似于人类免疫缺陷病毒Rev蛋白的核输出信号。有人提出，34 kDa的蛋白可能通过类似Rev的NES介导的输出途径促进腺病毒晚期mRNA的输出。我们测试了NES介导的RNA输出在腺病毒感染过程中的作用，发现它对于腺病毒晚期基因的表达并非必不可少。
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1
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J Virol. 2000 Jan;74(2):764-72. doi: 10.1128/jvi.74.2.764-772.2000.
2
Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.鉴定人类TAP的新型输入和输出信号，TAP是一种与D型逆转录病毒mRNA的组成型转运元件结合的蛋白质。
Mol Cell Biol. 1999 Sep;19(9):6306-17. doi: 10.1128/MCB.19.9.6306.
3
Cell biology. Snail mail to the nucleus.
Nature. 1999 May 20;399(6733):208-10. doi: 10.1038/20322.
4
RNA-binding activity of the E1B 55-kilodalton protein from human adenovirus type 5.来自人5型腺病毒的E1B 55千道尔顿蛋白的RNA结合活性
J Virol. 1998 Nov;72(11):9374-9. doi: 10.1128/JVI.72.11.9374-9379.1998.
5
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J Virol. 1998 Oct;72(10):7960-71. doi: 10.1128/JVI.72.10.7960-7971.1998.
6
Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1.细霉素B通过直接结合CRM1来抑制信号介导的核输出。
Exp Cell Res. 1998 Aug 1;242(2):540-7. doi: 10.1006/excr.1998.4136.
7
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8
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9
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Cell. 1997 Sep 19;90(6):967-70. doi: 10.1016/s0092-8674(00)80361-x.
10
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