Jewgenow K, Rohleder M, Wegner I
Institute for Zoo Biology and Wildlife Research, PF 601103, D-10252 Berlin, Germany.
J Reprod Fertil. 2000 May;119(1):15-23.
Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.
尽管付出了诸多努力，但在世界各地的城市地区，控制野猫种群的繁殖仍是一个难题。免疫避孕是一种很有前景的方法；因此，本研究探讨了广泛使用的猪透明带蛋白（pZP）用于家猫避孕的适用性。从猪和猫的卵巢中获得的纯化透明带蛋白被用于在兔子体内产生高度特异性的抗血清。在兔子或狮子体内产生的针对pZP的抗体，对体外精子结合（猫精子与猫卵母细胞）或猫的体外受精均无有效抑制作用，而在兔子体内产生的针对猫透明带蛋白（fZP）的抗体则表现出对体外受精的剂量依赖性抑制作用。卵巢的免疫电泳、酶联免疫吸附测定和免疫组织学证实了这些结果，显示抗fZP血清与fZP有交叉反应，与pZP的交叉反应较弱，但抗pZP血清与fZP无相互作用。得出的结论是，猫和猪的透明带表达的共同抗原决定簇数量极少，使得在猫身上使用pZP疫苗存在疑问。如果能够实现免疫原性，基于猫透明带决定簇的避孕疫苗将是控制野猫繁殖的更好选择。
