Granoff D M, Nankervis G A
Am J Dis Child. 1976 Nov;130(11):1211-4. doi: 10.1001/archpedi.1976.02120120045006.
The techniques of serum countercurrent immunoelectrophoresis, hemagglutinating (HA), and bactericidal (BC) antibody determinations were applied to seven patients with Haemophilus influenzae type B cellulitis. Capsular antigen was detected (greater than or equal to 10 ng/ml) in two patients at the time of admission and was found in two additional patients two days following hospitalization. Antibody responses in the patients with cellulitis were deficient, similar to those in children under 2 years of age with H influenzae type B meningitis. One of the patients with cellulitis had HA and BC antibody present in convalescent serum, and two additional patients demonstrated BC activity. The clinical presentations of our patients were typical except that violaceous discoloration of overlying skin was noted in only one of the seven. Two additional patients had atypical facial swelling that closely resembled angioneurotic edema and initially led to incorrect diagnosis. Countercurrent immunoelectrophoresis may be helpful in the diagnosis of patients with Haemophilus cellulitis, particularly if serial serum determinations are performed. Bacterial cultures obtained from blood and aspirates of the lesion appear to be more sensitive in determining the cause of H influenzae type B cellulitis.
血清对流免疫电泳、血凝（HA）及杀菌（BC）抗体测定技术应用于7例B型流感嗜血杆菌蜂窝织炎患者。2例患者入院时检测到荚膜抗原（大于或等于10 ng/ml），另外2例患者在住院两天后检测到。蜂窝织炎患者的抗体反应不足，类似于2岁以下患B型流感嗜血杆菌脑膜炎的儿童。1例蜂窝织炎患者恢复期血清中有HA和BC抗体，另外2例患者表现出BC活性。我们患者的临床表现典型，不过7例中只有1例出现了覆盖皮肤的紫蓝色变色。另外2例患者有非典型面部肿胀，酷似血管神经性水肿，最初导致了误诊。对流免疫电泳可能有助于诊断流感嗜血杆菌蜂窝织炎患者，特别是如果进行系列血清测定的话。从血液和病变部位抽吸物中获得的细菌培养物在确定B型流感嗜血杆菌蜂窝织炎病因方面似乎更敏感。
