Granoff D M, Rockwell R
Infect Immun. 1978 Jun;20(3):705-13. doi: 10.1128/iai.20.3.705-713.1978.
Infant rats inoculated intraperitoneally with Haemophilus influenzae type b develop bacteremia and meningitis. Rats were infected at 10 to 12 days of age and studied for the development of serum anticapsular antibody and bactericidal and opsonizing activity. Seven and 11 weeks after inoculation, convalescent animals showed a higher frequency of anticapsular antibody responses than uninfected controls, but 35 to 40% of the infected group had undetectable levels of anticapsular antibody (<0.10 mug/ml). In contrast, all of the convalescent animals, but none of the controls, showed moderate titers of serum bactericidal activity; and bactericidal activity persisted after absorption of the convalescent sera with type b capsule. Bactericidal activity was detected primarily in the eluted fraction corresponding to a molecular weight of 150,000 and was present in the offspring of convalescent females. Offspring of convalescent females were protected against challenge with H. influenzae type b, and control offspring could also be protected by passive immunization with convalescent serum which lacked detectable anticapsular antibody. Convalescent serum samples efficiently opsonized H. influenzae type b, and this activity persisted after absorption of the serum with capsular antigen. These data are consistent with the hypothesis that antibody to the noncapsular surface antigens of H. influenzae type b play an important role in host defenses.
经腹腔接种b型流感嗜血杆菌的幼鼠会发生菌血症和脑膜炎。这些幼鼠在10至12日龄时受到感染，并对血清抗荚膜抗体的产生以及杀菌和调理活性进行研究。接种后7周和11周，恢复期动物产生抗荚膜抗体反应的频率高于未感染的对照组，但感染组中有35%至40%的动物抗荚膜抗体水平检测不到（<0.10微克/毫升）。相比之下，所有恢复期动物的血清均显示出中等滴度的杀菌活性，而对照组则没有；用b型荚膜吸收恢复期血清后，杀菌活性仍然存在。杀菌活性主要在分子量为150,000的洗脱组分中检测到，并且存在于恢复期雌性动物的后代中。恢复期雌性动物的后代对b型流感嗜血杆菌的攻击具有抵抗力，对照组的后代也可以通过用缺乏可检测到的抗荚膜抗体的恢复期血清进行被动免疫来获得保护。恢复期血清样本能够有效地调理b型流感嗜血杆菌，并且在用荚膜抗原吸收血清后这种活性仍然存在。这些数据与以下假设一致，即针对b型流感嗜血杆菌非荚膜表面抗原的抗体在宿主防御中起重要作用。
