Granoff D M, Nankervis G A
J Infect Dis. 1977 Aug;136(2):292-6. doi: 10.1093/infdis/136.2.292.
The kinetics of bacteremia and capsular antigenemia in infant rats infected with Haemophilus influenzae type b were measured by quantitative bacterial counts in blood and counterimmunoelectrophoresis of plasma. After intraperitoneal inoculation with 10(4) colony-forming units (cfu) of H. influenzae type b, bacteremia was detected in 100% of animals at 12 hr after inoculation (mean, 16,500 cfu/ml) and by two days exceeded 10(5) cfu/ml in most animals. Despite these high levels of bacteremia, capsular antigen was detected infrequently during the early phase of experimental infection; it was present in 20% of animals at 12 hr and in 50% at one day. Peak levels of antigen in blood occurred two to three days after inoculation and coincided with the histologic appearance of meningitis. Thereafter, the frequency of antigenemia declined and paralleled the decline in quantitative bacterial counts in blood. Since detection of antigen was dependent on the occurrence of prolonged infection, counterimmunoelectrophoresis proved to be an insensitive method for early diagnosis.
通过对血液进行定量细菌计数以及对血浆进行对流免疫电泳，测定了感染b型流感嗜血杆菌的幼鼠的菌血症和荚膜抗原血症的动力学。用10⁴个b型流感嗜血杆菌菌落形成单位（cfu）进行腹腔接种后，100%的动物在接种后12小时检测到菌血症（平均为16,500 cfu/ml），到两天时，大多数动物的菌血症超过10⁵ cfu/ml。尽管菌血症水平很高，但在实验感染的早期阶段很少检测到荚膜抗原；12小时时20%的动物中存在荚膜抗原，一天时50%的动物中存在。血液中抗原的峰值水平在接种后两到三天出现，与脑膜炎的组织学表现一致。此后，抗原血症的频率下降，并与血液中定量细菌计数的下降平行。由于抗原的检测依赖于长时间感染的发生，对流免疫电泳被证明是早期诊断的一种不敏感方法。
