体外X射线照射后人淋巴细胞中10号染色体重排的高发生率。
High prevalence of chromosome 10 rearrangements in human lymphocytes after in vitro X-ray irradiation.
作者信息
Scarpato R, Lori A, Tomei A, Cipollini M, Barale R
机构信息
Dipartimento di Scienze dell'Uomo e dell'Ambiente, Università degli Studi di Pisa, Italy.
出版信息
Int J Radiat Biol. 2000 May;76(5):661-6. doi: 10.1080/095530000138321.
PURPOSE
To evaluate the chromosome symmetric or asymmetric rearrangement (CR) frequency for chromosome 10 compared to chromosomes 1 and 3 induced in vitro in human lymphocytes by low doses of X-rays.
MATERIALS AND METHODS
Blood samples obtained from three young and healthy volunteers were irradiated in G0 with 0.25, 0.50 and 1 Gy X-rays. Chromosome painting analysis was used on preparations of peripheral lymphocytes for the identification of CR.
RESULTS
It was found that radiation-induced CR levels were nonrandomly distributed among the three painted chromosomes. Chromosome 10 CR frequencies were significantly greater than those involving chromosomes 1 (at all the doses tested) or 3 (at 0.25 and 1 Gy), with frequency ratios ranging from 2.2 to 5.2.
CONCLUSIONS
In comparison to chromosomes 1 and 3, chromosome 10 appeared to be involved in exchanging at a significantly greater extent than expected according to its DNA content.
目的
评估低剂量X射线体外诱导人淋巴细胞时，与1号和3号染色体相比，10号染色体的染色体对称或不对称重排（CR）频率。
材料与方法
采集三名年轻健康志愿者的血液样本，在G0期用0.25、0.50和1 Gy的X射线进行照射。对外周血淋巴细胞制剂进行染色体涂染分析以鉴定CR。
结果
发现辐射诱导的CR水平在三条涂染染色体之间呈非随机分布。10号染色体的CR频率显著高于涉及1号染色体（在所有测试剂量下）或3号染色体（在0.25和1 Gy剂量下）的频率，频率比在2.2至5.2之间。
结论
与1号和3号染色体相比，10号染色体似乎参与交换的程度明显高于根据其DNA含量预期的程度。
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