D'Amato R F, Pisano M A
Antonie Van Leeuwenhoek. 1976;42(3):299-308. doi: 10.1007/BF00394127.
A chemically defined medium was developed for the biosynthesis of cephalosporin C by Paecilomyces persicinus Nicot strain P-10. Glucose served as the major carbon source and nitrogen was supplied by five amino acids, L-arginine, L-aspartic acid, L-glutamic acid, glycine and DL-methionine. Omission of any of the first four diminished or prevented production of cephalosporin C; omission of methionine did not. Methionine is not critical for the production of cephalosporin C in this defined medium. Production of the antibiotic was affected by the concentrations of inorganic salts employed. Biotin was required for growth and cephalosporin C synthesis. The addition of L-lysine precursors to the medium did not influence cephalosporin C levels and L-lysine itself inhibited antibiotic production. Known precursors of beta-lactam antibiotics as well as oleic acid did not affect biosynthesis of cephalosporin C. Chemical changes occurring in the defined medium revealed that glucose was efficiently utilized after 96 hours incubation whereas total soluble nitrogen levels increased following an initial sharp decrease. Mycelial weight and cephalosporin C production were both maximal after 96 hours incubation. Mycelial nitrogen was highest after 48 hours incubation whereas mycelial lipid levels were greatest after 72 hours.
开发了一种化学成分明确的培养基，用于由嗜虫拟青霉Nicot菌株P - 10生物合成头孢菌素C。葡萄糖作为主要碳源，氮由五种氨基酸提供，即L - 精氨酸、L - 天冬氨酸、L - 谷氨酸、甘氨酸和DL - 蛋氨酸。省略前四种氨基酸中的任何一种都会减少或阻止头孢菌素C的产生；省略蛋氨酸则不会。在这种特定培养基中，蛋氨酸对头孢菌素C的产生并不关键。抗生素的产生受到所用无机盐浓度的影响。生物素是生长和头孢菌素C合成所必需的。向培养基中添加L - 赖氨酸前体不会影响头孢菌素C的水平，而L - 赖氨酸本身会抑制抗生素的产生。已知的β - 内酰胺抗生素前体以及油酸均不影响头孢菌素C的生物合成。在特定培养基中发生的化学变化表明，培养96小时后葡萄糖被有效利用，而总可溶性氮水平在最初急剧下降后有所增加。培养96小时后菌丝体重量和头孢菌素C产量均达到最大值。培养48小时后菌丝体氮含量最高，而培养72小时后菌丝体脂质水平最高。
