Malowitz R, Pisano M A
Appl Environ Microbiol. 1982 Apr;43(4):916-23. doi: 10.1128/aem.43.4.916-923.1982.
The carbohydrate composition of the cell walls of Paecilomyces persicinus P-10 M1 was monitored daily for 6 days to detect any changes during growth and cephalosporin C production. Walls were isolated after mechanical breakage, sonication, and exposure to detergent. Major quantitative changes in cell wall carbohydrate composition accompanied a decrease in both cell weight and antibiotic production. Glucosamine content remained relatively constant in the 24- to 96-h cell walls and increased markedly in the 120- and 144-h preparations. The non-nitrogenous carbohydrate cell wall component, however, decreased significantly in the 48- and 120-h cell walls. Gas-liquid chromatographic analysis of the non-nitrogenous carbohydrate cell well fraction revealed the presence of glucose, the major component, mannose, galactose, and minute quantities of arabinose. Except for glucose, which was found to decrease moderately in the 120- and 144-h cell walls, the neutral sugars did not vary significantly with time.
对桃色拟青霉P - 10 M1细胞壁的碳水化合物组成进行了为期6天的每日监测，以检测其在生长和头孢菌素C产生过程中的任何变化。通过机械破碎、超声处理和洗涤剂处理后分离细胞壁。细胞壁碳水化合物组成的主要定量变化伴随着细胞重量和抗生素产量的下降。在24至96小时的细胞壁中，氨基葡萄糖含量相对保持恒定，而在120和144小时的制剂中显著增加。然而，非含氮碳水化合物细胞壁成分在48和120小时的细胞壁中显著减少。对非含氮碳水化合物细胞壁部分的气液色谱分析显示存在葡萄糖（主要成分）、甘露糖、半乳糖和微量阿拉伯糖。除了在120和144小时的细胞壁中发现葡萄糖适度减少外，中性糖随时间没有显著变化。
