Nielsen V G, Geary B T, Baird M S
Department of Anesthesiology, The University of Alabama at Birmingham, 35249, USA.
Anesth Analg. 2000 Jul;91(1):35-9. doi: 10.1097/00000539-200007000-00007.
The contribution of platelets and soluble clotting components to clot strength has been the focus of several clinical studies using thromboelastography; it would, therefore, be beneficial to develop an animal model with which to mechanistically approach hemostatic disorders. Thus, we proposed to determine if the contribution of platelet function (G(P), dyne/cm(2)) and soluble components of the coagulation pathway to total clot strength (G(T)) in rabbits were similar to those in humans. Blood was sampled from the ear arteries of conscious rabbits (n = 12); 350 microL of the blood was placed in a thromboelastograph. Ten microliters of normal saline, cytochalasin D (an inhibitor of microtubule function, 10 microM final concentration), or tissue factor (a potent stimulator of platelet function, 0.00625% final concentration) was added to the blood sample, and thromboelastography performed for 1 h. The G(T) (mean +/- SD) was significantly (P < 0.001) different among samples exposed to normal saline, cytochalasin D, or tissue factor, with G(T) values of 7238 +/- 1432, 937 +/- 372, and 16,556 +/- 3314, respectively. G(P) was responsible for 87% and 94% of G(T) in the absence or presence of tissue factor, respectively. G(P) did not significantly correlate with platelet concentration in the absence or presence of tissue factor. The contribution of G(P) to G(T) is similar to that observed in humans.
Rabbits may serve as a model of hemostasis that closely approximates human situations to mechanistically determine the etiology of coagulopathy. The contribution of platelet function to total clot strength is similar to that observed in humans.
血小板和可溶性凝血成分对血凝块强度的作用一直是几项使用血栓弹力图的临床研究的重点；因此，开发一种动物模型以从机制上研究止血障碍将是有益的。因此，我们提议确定兔血小板功能（G(P)，达因/平方厘米）和凝血途径的可溶性成分对总血凝块强度（G(T)）的作用是否与人类相似。从清醒兔（n = 12）的耳动脉采集血液；将350微升血液置于血栓弹力图仪中。向血样中加入10微升生理盐水、细胞松弛素D（微管功能抑制剂，终浓度10微摩尔）或组织因子（血小板功能的强效刺激剂，终浓度0.00625%），并进行1小时的血栓弹力图检测。暴露于生理盐水、细胞松弛素D或组织因子的样本之间的G(T)（平均值±标准差）有显著差异（P < 0.001），G(T)值分别为7238±1432、937±372和16556±3314。在不存在或存在组织因子的情况下，G(P)分别占G(T)的87%和94%。在不存在或存在组织因子的情况下，G(P)与血小板浓度均无显著相关性。G(P)对G(T)的作用与在人类中观察到的相似。
兔可作为一种止血模型，与人类情况非常接近，以从机制上确定凝血病的病因。血小板功能对总血凝块强度的作用与在人类中观察到的相似。
