FtsK的所有主要区域对于染色体二聚体的拆分都是必需的。
All major regions of FtsK are required for resolution of chromosome dimers.
作者信息
Boyle D S, Grant D, Draper G C, Donachie W D
机构信息
Institute of Cell & Molecular Biology, University of Edinburgh, Scotland.
出版信息
J Bacteriol. 2000 Jul;182(14):4124-7. doi: 10.1128/JB.182.14.4124-4127.2000.
Abstract
Resolution of chromosome dimers, by site-specific recombination between dif sites, is carried out in Escherichia coli by XerCD recombinase in association with the FtsK protein. We show here that a variety of altered FtsK polypeptides, consisting of the N-terminal (cell division) domain alone or with deletions in the proline-glutamine-rich part of the protein, or polypeptides consisting of the C-terminal domain alone are all unable to carry out dif recombination. Alteration of the putative nucleotide-binding site also abolishes the ability of FtsK to carry out recombination between dif sites.
摘要
在大肠杆菌中，通过dif位点之间的位点特异性重组来解决染色体二聚体的问题，这是由XerCD重组酶与FtsK蛋白共同完成的。我们在此表明，多种改变的FtsK多肽，单独由N端（细胞分裂）结构域组成，或在蛋白质富含脯氨酸 - 谷氨酰胺的部分有缺失，或单独由C端结构域组成，都无法进行dif重组。假定的核苷酸结合位点的改变也消除了FtsK在dif位点之间进行重组的能力。
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FtsK is a bifunctional protein involved in cell division and chromosome localization in Escherichia coli.FtsK是一种双功能蛋白，参与大肠杆菌的细胞分裂和染色体定位。
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FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response.FtsK是一种必需的细胞分裂蛋白，定位于隔膜，并作为SOS反应的一部分被诱导产生。
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