FtsK是一种必需的细胞分裂蛋白，定位于隔膜，并作为SOS反应的一部分被诱导产生。

FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response.

作者信息

Wang L, Lutkenhaus J

机构信息

Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA.

出版信息

Mol Microbiol. 1998 Aug;29(3):731-40. doi: 10.1046/j.1365-2958.1998.00958.x.

DOI:10.1046/j.1365-2958.1998.00958.x

PMID:9723913

Abstract

The role of ftsK in the growth of Escherichia coli was examined by turning off its expression. This resulted in smooth filaments without constrictions, indicating that FtsK was required at an early step in septation. Consistent with this, FtsK was found to localize to the septum in 70% of the cells, indicating that it was recruited relatively early in this process. FtsK localization required the function of FtsZ and FtsA but not FtsI and FtsQ. Consistent with this, Z rings were present in FtsK-depleted filaments. Subcellular localization of FtsK confirmed that it was a membrane protein. Only the first 202 amino acids of FtsK were essential for its role in membrane localization, cell division and viability. The expression of ftsK increased as part of the SOS response, and increased expression of ftsK conferred increased resistance to DNA damage.

摘要

通过关闭ftsK的表达来研究其在大肠杆菌生长中的作用。这导致形成了没有缢缩的光滑细丝，表明FtsK在隔膜形成的早期阶段是必需的。与此一致的是，发现70%的细胞中FtsK定位于隔膜，这表明它在此过程中相对较早地被募集。FtsK的定位需要FtsZ和FtsA的功能，但不需要FtsI和FtsQ的功能。与此一致的是，在FtsK缺失的细丝中存在Z环。FtsK的亚细胞定位证实它是一种膜蛋白。FtsK仅前202个氨基酸对于其在膜定位、细胞分裂和生存能力中的作用至关重要。ftsK的表达作为SOS反应的一部分而增加，并且ftsK表达的增加赋予了对DNA损伤的抗性增加。