Mita T, Bujadoux M, Sabolovic D, Oth D
Biomedicine. 1976 Apr 20;25(2):52-5.
Murine thymus cells were caltivated up to 10 days in a semi-solid medium. Both macroscopically visible colonies and DNA synthesis (identified by 3H-thymidine incorporation) were counted with and without added sera. According to their origins the sera exerted different modifications of the cultivated cells behaviour. Changes of colony number and of thymidine incorporation were quite independent on each other.
将小鼠胸腺细胞在半固体培养基中培养长达10天。在添加和不添加血清的情况下,对肉眼可见的集落和DNA合成(通过3H-胸腺嘧啶核苷掺入鉴定)进行计数。根据血清来源的不同,其对培养细胞的行为产生不同的影响。集落数量的变化和胸腺嘧啶核苷掺入的变化彼此完全独立。