Ruscetti F W, Chervenick P A
J Clin Invest. 1975 Mar;55(3):520-7. doi: 10.1172/JCI107958.
Colony-stimulating activity (CSA) is essential for in vitro differentiation of bone marrow cells into colonies of granulocytes and mononuclear cells. While blood monocytes and macrophages are a major source of CSA, recent studies have indicated that CSA may be produced by lymphocytes responding to immunologic stimulation. Lymphocytes, purified from spleens and thymuses of mice by glass wool columns, were incubated in CMRL-1066 medium with fetal calf serum in vitro. Lymphocytes from the thumus and spleen released CSA when cultured in vitro, with peak levels of CSA observed after 7 days of incubation. Stimulation of cultures with phytohemagglutinin, concanavalin A, or pokeweed mitogen resulted in a 2-5-fold increase in CSA release, with peak levels of CSA released after 4 days of incubation. Thymus-dependent lymphocytes were responsible for the release of CSA from unstimulated and mitogen-stimulated cultures, since the incubation of these cultures with rabbit anti-mouse T cell sera abolished their ability to release CSA. Anti-mouse B cell sera had no effect on the ability of lymphocyte cultures to release CSA. These studies suggest that thymocytes and thymus-derived lymphocytes can release CSA in vitro and may be responsible for the increase in CSA observed in certain immunologic reactions.
集落刺激活性（CSA）对于骨髓细胞在体外分化为粒细胞和单核细胞集落至关重要。虽然血液单核细胞和巨噬细胞是CSA的主要来源，但最近的研究表明，CSA可能由对免疫刺激作出反应的淋巴细胞产生。通过玻璃棉柱从小鼠脾脏和胸腺中纯化的淋巴细胞，在含有胎牛血清的CMRL-1066培养基中进行体外培养。来自胸腺和脾脏的淋巴细胞在体外培养时会释放CSA，培养7天后观察到CSA的峰值水平。用植物血凝素、刀豆蛋白A或商陆有丝分裂原刺激培养物，会导致CSA释放增加2至5倍，培养4天后观察到CSA的峰值释放水平。胸腺依赖性淋巴细胞负责在未刺激和有丝分裂原刺激的培养物中释放CSA，因为用兔抗小鼠T细胞血清孵育这些培养物会消除它们释放CSA的能力。抗小鼠B细胞血清对淋巴细胞培养物释放CSA的能力没有影响。这些研究表明，胸腺细胞和胸腺来源的淋巴细胞在体外可释放CSA，并且可能是某些免疫反应中观察到的CSA增加的原因。
