Kameya T, Kuramoto H, Suzuki K, Kenjo T, Oshikiri T, Hayashi H, Itakura M
Cancer Res. 1975 Aug;35(8):2025-32.
A gastric choriocarcinoma cell line synthesizing human chorionic gonadotropin (HCG) was established in 1971 by Oboshi et al. and was found to possess human placental alkaline phosphatase. The present paper also deals with the relationship between the cell growth and HCG secretion and with cellular localization of HCG and human placental alkaline phosphatase by cytochemical and ultrastructural methods. This cell line was found to secrete HCG during cellular proliferation, with the maximum secretion in the stationary phase (about 1 muIU/cell/48 hr), and the hormone could be detected in a small proportion of mono- and/or multinuclear cells in both logarithmic and stationary phases. The organ-specific, heat-stable, L-phenylalanine-sensitive, immunoreactive human placental alkaline phosphatase was localized on the cell membrane of many cells. Ultrastructurally, the line consisted mainly of cytotrophoblastic and intermediate cells in the process of syncytial formation, with more or less squamous metaplasia. From these findings it was concluded that the cell line maintained the properties of trophoblastic cells from morphological and functional aspects, i.e., it was a cell line with two distinct marker substances.
1971年，小星等建立了一株能合成人绒毛膜促性腺激素（HCG）的胃绒毛膜癌细胞系，并发现该细胞系具有人胎盘碱性磷酸酶。本文还通过细胞化学和超微结构方法研究了细胞生长与HCG分泌之间的关系以及HCG和人胎盘碱性磷酸酶的细胞定位。发现该细胞系在细胞增殖过程中分泌HCG，在静止期分泌量最大（约1毫国际单位/细胞/48小时），在对数期和静止期的一小部分单核和/或多核细胞中可检测到该激素。器官特异性、热稳定、L-苯丙氨酸敏感、免疫反应性人胎盘碱性磷酸酶定位于许多细胞的细胞膜上。超微结构上，该细胞系主要由正在形成合体滋养层的细胞滋养层细胞和中间细胞组成，或多或少有鳞状化生。从这些发现可以得出结论，该细胞系从形态和功能方面保持了滋养层细胞的特性，即它是一个具有两种不同标志物的细胞系。
