Cao J X, Krell P J, Nagy E
Department of Pathobiology, University of Guelph, Ontario, Canada.
Virus Genes. 2000;20(2):135-7. doi: 10.1023/a:1008114430408.
Two transcription products were found for the open reading frame (ORF) RTL1 located near the right terminus of the fowl adenovirus type-8 genome. The larger transcript, which was transcribed mostly during the early stage of the virus infection, contains the complete sequence (933 nucleotides) of the predicted ORF from the genomic DNA sequence encoding a 311 amino acid (aa) polypeptide. In contrast, the shorter transcript, which was more predominant at the late stage of the infection, was missing 580 nucleotides (from nucleotide 117 to 696). A premature stop codon was introduced at 210 nucleotides downstream from the start codon and the shorter transcript would encode a 70 aa polypeptide. This observation indicates that the ORF RTL1 may produce two different proteins, which function differently at different stages of the virus infection. Another possibility is that the virus may use alternative splicing as a mechanism to control the expression of the ORF, since the spliced transcript was prematurely terminated at the late stage of the infection.
在禽腺病毒8型基因组右末端附近的开放阅读框（ORF）RTL1中发现了两种转录产物。较大的转录本主要在病毒感染早期转录，包含来自基因组DNA序列的预测ORF的完整序列（933个核苷酸），该序列编码一个311个氨基酸（aa）的多肽。相比之下，较短的转录本在感染后期更为突出，缺失了580个核苷酸（从第117个核苷酸到696个核苷酸）。在起始密码子下游210个核苷酸处引入了一个提前终止密码子，较短的转录本将编码一个70个氨基酸的多肽。这一观察结果表明，ORF RTL1可能产生两种不同的蛋白质，它们在病毒感染的不同阶段发挥不同的功能。另一种可能性是，病毒可能利用可变剪接作为控制ORF表达的机制，因为剪接后的转录本在感染后期提前终止。
