Cao J X, Krell P J, Nagy E
Department of Pathobiology, University of Guelph, Ontario, Canada.
J Gen Virol. 1998 Oct;79 ( Pt 10):2507-16. doi: 10.1099/0022-1317-79-10-2507.
Fowl adenovirus (FAdV) type 1, CELO strain has no homologies to mastadenovirus E1A, E1B, E3 and E4, which regulate virus gene expression, DNA replication and virus-host interaction. Similarly, the right 5 kb and left 15 kb ends of CELO virus DNA are non-homologous to mastadenoviruses. To compare CELO virus with another FAdV, 7.5 kb of the left and 17 kb of the right ends of FAdV type 8 (strain A-2A) were sequenced and nine and 17 open reading frames (ORFs), respectively, were found. This FAdV-8 genome was similar to CELO virus in that (1) the central region contained the major structural protein genes including the fibre, pVIII, 100K, late 33K and pIVa2 genes, which were in the same order as in mastadenoviruses, (2) no homologues of mastadenovirus E1A, E1B, E3 and E4 were found in the ends, and (3) the left 6 kb and the right 13 kb ends showed no homology to mastadenoviruses. Several genomic features were unique to FAdV-8 compared to CELO virus. FAdV-8 contained one fibre gene in contrast to two in CELO virus. Three of eight unassigned ORFs in the left and five of 13 unassigned ORFs in the right ends were unique compared to CELO virus. Two sets of tandem repeats, one with five identical 33 bp repeats and the other with more than ten identical 135 bp repeats, mapped between 4.5 and 7.5 kb from the right terminus. No virus-associated RNA gene was found. Fifteen of 16 unique FAdV-8 ORFs tested were, as determined by RT-PCR, transcribed early.
1型禽腺病毒（FAdV）CELO株与调控病毒基因表达、DNA复制及病毒-宿主相互作用的哺乳动物腺病毒E1A、E1B、E3和E4没有同源性。同样，CELO病毒DNA的右侧5 kb末端和左侧15 kb末端与哺乳动物腺病毒也没有同源性。为了将CELO病毒与另一种禽腺病毒进行比较，对8型禽腺病毒（A-2A株）的左侧7.5 kb和右侧17 kb末端进行了测序，分别发现了9个和17个开放阅读框（ORF）。该8型禽腺病毒基因组与CELO病毒相似之处在于：（1）中央区域包含主要结构蛋白基因，包括纤维蛋白、pVIII、100K、晚期33K和pIVa2基因，其排列顺序与哺乳动物腺病毒相同；（2）在末端未发现与哺乳动物腺病毒E1A、E1B、E3和E4的同源物；（3）左侧6 kb和右侧13 kb末端与哺乳动物腺病毒无同源性。与CELO病毒相比，8型禽腺病毒有几个独特的基因组特征。与CELO病毒有两个纤维蛋白基因不同，8型禽腺病毒含有一个纤维蛋白基因。与CELO病毒相比，左侧8个未分配的ORF中有3个以及右侧13个未分配的ORF中有5个是独特的。在距离右末端4.5至7.5 kb之间定位了两组串联重复序列，一组有5个相同的33 bp重复序列，另一组有10多个相同的135 bp重复序列。未发现病毒相关RNA基因。通过逆转录聚合酶链反应（RT-PCR）测定，所检测的16个8型禽腺病毒独特ORF中有15个在早期转录。
