Fliegerova K, Pazoutova S, Pristas P, Flint H J
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 10-Uhrineves, Prague, 104 00, Czech Republic.
Plasmid. 2000 Jul;44(1):94-9. doi: 10.1006/plas.2000.1464.
Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16,525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21.
来自反刍月形单胞菌乳酸亚种JW13菌株的质粒pJW1已克隆到大肠杆菌载体pBluescriptSK(-)中并进行了全序列测定。该质粒仅1410 bp，总GC含量为42.2%。对序列数据的计算机分析揭示了一个单一的开放阅读框（ORF1，146个氨基酸，分子量16,525.5 Da），其编码一种假定的复制蛋白，该蛋白与嗜淀粉瘤胃杆菌质粒pRAO1的Rep蛋白相似。ORF1之后是一个长的富含AT（75%）的区域以及一个富含正向和反向重复序列的区域。DNA序列比较显示存在一个短的（<250 bp）DNA片段，该片段在包括pJDB21在内的几个小反刍月形单胞菌质粒之间高度保守。
