Braun R D, Fisher T C, Meiselman H J, Hatchell D L
Durham VAMC, North Carolina, USA.
Microcirculation. 1996 Sep;3(3):271-8. doi: 10.3109/10739689609148301.
Polymorphonuclear leukocytes (PMN) have been found to be less deformable in humans with non-insulin-dependent diabetes. It has therefore been hypothesized that white blood cells (WBC) may affect the development of diabetic microangiopathy. This study was designed to determine whether PMN or small and large lymphocytes were less deformable in a large animal model of diabetes-chronically hyperglycemic pancreatectomized cats.
Venous blood was withdrawn from 13 normal and 14 diabetic cats. The diabetic cats had been kept in poor metabolic control since their pancreatectomy (7-113 months before this study). Blood cell counts, hemoglobin concentration, hematocrit, erythrocyte volume, and WBC differential counts were obtained from the blood samples. Purified mononuclear WBC and PMN fractions were obtained by separating the blood on a discontinuous Percoll gradient. The deformability of each cell fraction was determined using a Cell Transit Analyzer (ABX, Montpellier, France) that measures the transit time of cells through 7.5-microns pores. By varying the sampling rate of the CTA and the pressure difference across the filter, the subpopulations of the mononuclear fractions (small and large lymphocytes) could be identified and each subpopulation analyzed separately.
Median transit times for the PMN and small lymphocytes were significantly greater for the diabetic cats, but no difference was found in the large lymphocyte fractions.
These results are in accordance with the finding that PMN are less deformable in humans with diabetes. We also showed that the small lymphocytes from diabetic cats have prolonged transit times. The results suggest that PMN may contribute to the development of microangiopathies like diabetic retinopathy. Diabetic cats may prove useful for testing potential therapies to improve WBC deformability.
已发现多形核白细胞（PMN）在非胰岛素依赖型糖尿病患者中变形能力较差。因此，有人推测白细胞（WBC）可能会影响糖尿病微血管病变的发展。本研究旨在确定在糖尿病的大型动物模型——长期高血糖的胰腺切除猫中，PMN或小淋巴细胞及大淋巴细胞的变形能力是否较差。
从13只正常猫和14只糖尿病猫中采集静脉血。这些糖尿病猫自胰腺切除后（本研究前7 - 113个月）代谢控制一直较差。从血样中获取血细胞计数、血红蛋白浓度、血细胞比容、红细胞体积和白细胞分类计数。通过在不连续的Percoll梯度上分离血液获得纯化的单核白细胞和PMN组分。使用细胞迁移分析仪（法国蒙比利埃ABX公司）测定每个细胞组分的变形能力，该分析仪测量细胞通过7.5微米孔径的迁移时间。通过改变CTA的采样率和过滤器两端的压差，可以识别单核组分的亚群（小淋巴细胞和大淋巴细胞）并分别分析每个亚群。
糖尿病猫的PMN和小淋巴细胞的中位迁移时间显著更长，但大淋巴细胞组分未发现差异。
这些结果与糖尿病患者中PMN变形能力较差的发现一致。我们还表明，糖尿病猫的小淋巴细胞迁移时间延长。结果表明，PMN可能促成糖尿病视网膜病变等微血管病变的发展。糖尿病猫可能被证明有助于测试改善白细胞变形能力的潜在疗法。
