急性髓性白血病的体外集落生长

In vitro colony growth of acute myelogenous leukemia.

作者信息

Dicke K A, Spitzer G, Cork A, Ahearn M J

出版信息

Nouv Rev Fr Hematol Blood Cells. 1976;17(1-2):125-37. doi: 10.1007/978-3-642-66312-3_8.

Abstract

Colony formation in vitro by marrow cells from patients with untreated acute myelogenous leukemia (AML) and from patients in AML relapse is infrequent using the standard Robinson assay. A newly developed culture system has been described in which marrow from AML patients in these disease stages form leukemic cell colonies. In this in vitro system, phytohaemagglutinin is the essential stimulator for colony formation. The leukemic origin of the colonies has been proven by ultrastructural morphology and cytogenetics. It appears that colony formation by leukemic cells in this system is predominantly independent from the leukocyte factor which is the main stimulator in the Robinson assay for growing colonies of marrow cells from haematologically normal individuals.

摘要

使用标准的罗宾逊检测法，未经治疗的急性髓性白血病（AML）患者以及处于AML复发期患者的骨髓细胞在体外形成集落的情况并不常见。已经描述了一种新开发的培养系统，处于这些疾病阶段的AML患者的骨髓在该系统中可形成白血病细胞集落。在这个体外系统中，植物血凝素是集落形成的关键刺激物。通过超微结构形态学和细胞遗传学已证实集落的白血病起源。在该系统中，白血病细胞形成集落似乎主要独立于白细胞因子，而白细胞因子是罗宾逊检测法中用于培养血液学正常个体骨髓细胞集落的主要刺激物。