Spitzer G, Schwarz M A, Dicke K A, Trujillo J M, McCredie K B
Nouv Rev Fr Hematol Blood Cells. 1976;17(1-2):149-59. doi: 10.1007/978-3-642-66312-3_10.
A technique for cloning myeloblastic leukemic cells in semi-solid agar, after prior PHA stimulation, was studied in all phases of chronic myeloid leukemia. Phytohemagglutinin responsive cells were found in blast crisis and accelerated phase. In a small number of patients in the benign phase of their disease, colony growth with PHA was detected. A group of patients with early acute leukemia was also examined by this technique. The incidence of PHA colonies appeared to correlate with the progression of the disease. The potential application of this assay for early detection of leukemic clones and possible prediction of the rapidity of progression of the leukemic process is discussed.
对一种在半固体琼脂中克隆髓母细胞性白血病细胞的技术进行了研究，该技术是在慢性髓性白血病的各个阶段，先经PHA刺激后进行的。在急变期和加速期发现了对植物血凝素（PHA）有反应的细胞。在少数处于疾病良性期的患者中，检测到了PHA刺激后的集落生长。还通过该技术对一组早期急性白血病患者进行了检查。PHA集落的发生率似乎与疾病进展相关。讨论了该检测方法在白血病克隆早期检测以及白血病进程进展速度预测方面的潜在应用。
