兔胰蛋白酶的纯化及特性
Purification and properties of rabbit trypsin.
作者信息
Johnson D A
出版信息
Biochim Biophys Acta. 1976 Dec 8;452(2):482-7. doi: 10.1016/0005-2744(76)90198-4.
PMID:1087571
Abstract
The purification of rabbit pancreatic trypsin (EC 3.4.21.4) by affinity chromatography on Trasylol-Sepharose is presented along with its physical, chemical and immunological relationship to other trypsins. The molecule is a single polypeptide chain, which immunologically cross-reacts with porcine trypsin, but not with rabbit acrosomal proteinase. Sequence homology with other mammalian trypsins is seen at the amino terminus.
摘要
介绍了通过抑肽酶-琼脂糖亲和层析法纯化兔胰腺胰蛋白酶（EC 3.4.21.4）及其与其他胰蛋白酶的物理、化学和免疫学关系。该分子为单条多肽链，在免疫学上与猪胰蛋白酶发生交叉反应，但与兔顶体蛋白酶不发生交叉反应。在氨基末端可观察到与其他哺乳动物胰蛋白酶的序列同源性。
Zotero 插件