Stambaugh R, Smith M
Science. 1974 Nov 22;186(4165):745-6. doi: 10.1126/science.186.4165.745.
Rabbit acrosomal proteinase from epididymal spermatozoa of 44 male rabbits was purified by subcellular fractionation, sucrose density gradient centrifugation, and electrofocusing; the specific activity of the purified product was 20,047 units per milligram, a value similar to that observed for pancreatic trypsins from various sources. The molecular weight determined from the amino acid analyses and ultracentrifugation was about 22,000. This rabbit acrosomal proteinase showed great similarity to pancreatic trypsin, especially to human pancreatic trypsin, both in the number of individual amino acids and in the total number of residues. This similarity was confirmed by an antigenic cross reaction between rabbit antiserum to bovine pancreatic trypsin with human, rabbit, rhesus monkey, and bull acrosotnal proteinase.
采用亚细胞分级分离、蔗糖密度梯度离心和等电聚焦法，从44只雄兔附睾精子中纯化出兔顶体蛋白酶；纯化产物的比活性为每毫克20,047单位，该值与从各种来源获得的胰蛋白酶的值相似。通过氨基酸分析和超速离心确定的分子量约为22,000。这种兔顶体蛋白酶在单个氨基酸数量和残基总数方面与胰蛋白酶，尤其是人胰蛋白酶非常相似。兔抗牛胰蛋白酶抗血清与人、兔、恒河猴和公牛顶体蛋白酶之间的抗原交叉反应证实了这种相似性。
