Hong S L, Patton G, Deykin D
Prostaglandins. 1979 Jan;17(1):53-9. doi: 10.1016/0090-6980(79)90074-1.
Methylcholanthrene transformed mouse fibroblast cells can be induced to synthesize prostaglandins by a short term incubation with various vasoactive agents including serum, bradykinin and thrombin or in response to mechanical detachment from the culture dish. The ability of the cells to synthesize prostaglandins upon stimulation changes during growth of the culture on the dish; the response is maximal on the first day after inoculation and decreased sharply thereafter. Feeding of the cells with fresh growth medium enhances prostaglandin production induced by all stimuli. The difference in the cell response during growth is probably not due to change of prostaglandin synthetase activity since the specific enzyme activities assayed with microsomal preparations of cells harvested from the first and third day culture are similar. However, analysis of the cellular content of arachidonic acid after saponification of the total lipid extract of cells harvested at different days of growth reveals that the level of arachidonic acid per cell during growth is parallel to the response to stimuli. It is maximal on the first day and decreases sharply on the second day and stays low on the third day. Our study suggests that the level of arachidonic acid in the cell governs the extent of prostaglandin synthesis upon stimulation.
用甲基胆蒽转化的小鼠成纤维细胞，通过与包括血清、缓激肽和凝血酶在内的各种血管活性物质短期孵育，或响应于从培养皿上机械分离，可被诱导合成前列腺素。在培养皿上培养期间，细胞在受到刺激时合成前列腺素的能力会发生变化；接种后的第一天反应最大，此后急剧下降。用新鲜生长培养基培养细胞可增强所有刺激诱导的前列腺素产生。生长过程中细胞反应的差异可能不是由于前列腺素合成酶活性的变化，因为用从第一天和第三天培养物中收获的细胞微粒体制剂测定的比酶活性相似。然而，对在不同生长天数收获的细胞总脂质提取物皂化后花生四烯酸的细胞含量分析表明，生长期间每个细胞的花生四烯酸水平与对刺激的反应平行。它在第一天最大，在第二天急剧下降，在第三天保持低水平。我们的研究表明，细胞中花生四烯酸的水平决定了刺激后前列腺素合成的程度。
